

Highlights
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
Description
Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
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Equipment | Microcentrifuge, vortex, magstand |
Sample Inactivation | TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. |
Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
Size Range | Total RNA ≥ 17 nt |
Yield | 10 µg RNA (binding capacity), ≥ 30 µl (elution volume) |
Q1: Is Direct-zol suitable for very small numbers of cells?
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q2: Is DNase I available for individual purchase?
All kit components are available for purchase separately.
Q3: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q4: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q5: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Q6: Is it possible to extract proteins with the Direct-zol RNA kits?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
Q10: Which phenol-based reagents are compatible with Direct-zol?
The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
“Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME!It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”
-A. Newhart (The Wistar Institute)
“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”
-Mohan K. (University of Illinois, Chicago)
“Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”
-Arjan V. (Indiana University)
Read MoreCat # | Name | Size | Price | |
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D4100-2-3 | MagBinding Beads | 3 ml | $66.00 | |
D4100-2-12 | MagBinding Beads | 12 ml | $125.00 | |
W1001-30 | DNase/RNase-Free Water | 30 ml | $22.00 | |
R1060-2-100 | RNA Prep Buffer | 100 ml | $122.00 | |
R1060-2-25 | RNA Prep Buffer | 25 ml | $40.00 | |
R2100-1-20 | Direct-zol Binding Buffer Concentrate | 20 ml | $84.00 | |
R2100-2-200 | Direct-zol MagBead PreWash | 200 ml | $174.00 | |
R2130-1-120 | MagBead DNA/RNA Wash 1 | 120 ml | $198.00 | |
R2130-1-30 | MagBead DNA/RNA Wash 1 | 30 ml | $63.00 | |
R2130-2-20 | MagBead DNA/RNA Wash 2 | 20 ml | $54.00 | |
R2130-2-80 | MagBead DNA/RNA Wash 2 | 80 ml | $171.00 | |
C2002 | Collection Plate | 2 Plates | $22.00 | |
C2003 | Elution Plate | 2 Plates | $19.00 | |
C2007-8 | 96-Well Plate Cover Foil | 8 Foils | $18.00 | |
R2050-1-200 | TRI Reagent | 200 ml | $219.00 | |
E1010-1-4 | DNA Digestion Buffer | 4 mL | $15.00 | |
E1010 | DNase I Set | 250 U | $56.00 |
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免疫组织化学技术按照标记物的种类可分为免疫荧光法、免疫酶法、免疫铁蛋白法、免疫金法及放射免疫自显影法等。免疫荧光细胞化学技术将已知抗体标上荧光素,以此作为探针检查细胞或组织内的相应抗原,在荧光显微镜下观察.当抗原抗体复合物中的荧光素受激发光的照射后会发出一定波长的荧光,从而可以确定组织中的抗原定位或定量.免疫酶细胞化学技术是目前免疫组织化学研究中最常用的技术.基本原理是先以酶标记的抗体与组织或细胞作用,然后加入酶的底物,生成有色的不溶性产物或具有一定电子密度的颗粒,通过光镜或电镜,对细胞或组织内的相应抗原进行定位或定性研究.免疫胶体金技术就是用胶体金标记一抗,二抗或其他的能特异性的结合免疫球蛋白的分子(如葡萄球菌A蛋白)等作为探针对组织或细胞内的抗原进行定性,定位或定量研究.由于胶体金的电子密度高,多用于免疫电镜的单标记或多标记的定位研究。近年来,随着免疫组织化学技术的发展和各种特异性抗体的出现,使许多疑难肿瘤得到了明确诊断。在常规肿瘤病理诊断中,5%-10%的病例单靠H.E.染色难以作出明确的形态学诊断。尤其是免疫组化在肿瘤诊断和鉴别诊断中的实用价值受到了普遍的认可,其在低分化或未分化肿瘤的鉴别诊断时,准确率可达50%-75%。
1.若都为正常,那么并未有太大问题,只要定期复查就可以了。
2.如果TGAb、TMAb、甲状腺功能均高,那么就是桥本氏甲状腺炎并甲亢,需要抗甲亢治疗。
3.如果TGAb、TMAb高,甲状腺功能下降,那么就是桥本氏甲状腺炎并甲减,需要进行甲减治疗。
4.如果TGAb、TMAb高,甲状腺功能正常,那么就是桥本氏甲状腺炎,无需特殊治疗,只要定期复查甲状腺功能就可能以了,不过这种情况有可能以后演变成甲减或者甲亢。
而国内的只有兔抗牛。
就二抗来说,国内的标记二抗质量也很不错。可以用。
慢性心功能不全的病因的防治
风湿性心瓣膜病在我国仍属慢性心力衰竭的常见病因。应用青霉素治疗链球菌感染,已使风湿热和风湿性心瓣膜病在发达国家基本绝迹。择期手术治疗风湿性心瓣膜病,有效地控制高血压以及积极防治冠脉病变与心肌缺血等病因治疗;消除心力衰竭的诱因如控制感染、避免体力过劳和精神应激等。可预防心力衰竭的发生。
收缩性心力衰竭的治疗
1、减轻心脏负荷包括减少体力活动和精神应激。严重者宜绝对卧床休息,在心功能逐步改善过程中,适当下床活动,以免卧床休息过久并发静脉血栓形成或肺炎。此外,应注意解除精神负担,必要时给予小量镇静剂。
2、限制钠盐摄入适当限制日常饮食中的钠盐摄入量,食盐量2~5g,忌盐腌制食物。应用利尿剂引起大量利尿时,钠盐限制不宜过严,以免发生低钠血症。
3、利尿剂的应用利尿剂通过抑制肾小管不同部位Na+重吸收,或增加肾小球Na+滤过,增进水、Na+排出,从而降低心室充盈压,减轻肺循环和(或)体循环瘀血所致临床症状,其疗效肯定,但对心力衰竭整体过程的影响(如生存率等)不明,长期应用利尿剂理论上可能产生下列不良作用:①降低心排血量,从而激活RAS,血浆肾素和醛固酮增高。②导致低钾血症。③降低糖耐量。④导致高尿酸血症。⑤导致高脂血症。⑥导致室性心律失常。目前利尿剂属治疗心力衰竭伴水、钠潴留患者的一线药物,大多与其他心力衰竭治疗药物(如地高辛、ACE抑制剂)联合应用,单纯舒张性心力衰竭者利尿剂宜慎用。
4、正性肌力药物的应用由于慢性心力衰竭患者心肌收缩性减弱,改善心肌收缩功能曾被认为是心力衰竭的首要治疗。正性肌力药物能使心室功能曲线左上移,增加每搏作功,降低心室充盈压,从而使扩大的心脏缩小。虽然在增加心肌收缩的同时也增加心肌能量消耗,但扩大的心脏缩小后,其心肌氧耗和冠脉血供分别较心脏扩大时降低和改善,心肌能量供需的不平衡因而并不加重,甚至有所减轻。正性肌力药减轻症状、改善运动耐量和心功分级的效果明显,但多中心随机对照慢性心力衰竭患者长期临床治疗试验结果表明除洋地黄外,大多具有增高病死率与室性心律失常发生率的倾向。ACEI则不仅减轻症状、改善运动耐量和心功分级的效果更显著,且能降低病死率和病残率。因而大多数临床医师选用ACEI作为与利尿剂联用,以治疗窦性心律的慢性心力衰竭患者。
西安医专附属医院 内科心血管专家杜志桢,1976年毕业于西安医科大学医疗系。2002年晋升为主任医师,从事内科心血管疾病临床工作30余年。对冠心病、心肌缺血、心绞痛及高血压病等治疗,积累了丰富的临床经验。对心脑血管相关疾病如高脂血症、心肾功能不全的治疗亦有较成熟经验。曾担任西安医学会心血管病学会委员。前列腺素E1治疗急性肾衰获市新技术项目奖(94年)。有关心血管病近10余篇论文公开发表。为东南大学《现代医学》杂志编委。
职称:主任医师
医院科室:西安医学专科学校附属医院心内科
擅长:冠心病、高血压病、心肌病、心肌炎、心力衰竭、心律失常、心肌梗塞、心肌缺血、心绞痛治疗。
治疗病名:冠心病、心梗、顽固心律失常、高血压心脏病、心衰、心绞痛、肺心病、风心病高脂血症、动脉硬化等。展开
1.若都为正常,那么并未有太大问题,只要定期复查就可以了。
2.如果TGAb、TMAb、甲状腺功能均高,那么就是桥本氏甲状腺炎并甲亢,需要抗甲亢治疗,那么,要注意戒碘饮食,避免吃一些含碘丰富的食物,如海产品等。
3.如果TGAb、TMAb高,甲状腺功能下降,那么就是桥本氏甲状腺炎并甲减,需要进行甲减治疗,饮食方面可以增加含碘丰富的食物摄入。
4.如果TGAb、TMAb高,甲状腺功能正常,那么就是桥本氏甲状腺炎,无需特殊治疗,只要定期复查甲状腺功能就可能以了,不过这种情况有可能以后演变成甲减或者甲亢。

