- SynonymIGF2,C11orf43,FLJ22066,FLJ44734,IGF-II,PP9974
- SourceBiotinylated Human IGF-II, Avitag,Fc Tag (IG2-H82F9) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Glu 91 (Accession # P01344-1).Predicted N-terminus: GlyRequest for sequence
- Molecular Characterization

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 35.3 kDa. As a result of glycosylation, the protein migrates as 40 kDa under reducing (R) condition, and 66 kDa under non-reducing (NR) condition (SDS-PAGE).
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Biotinylated Human IGF-II, Avitag,Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human IGFBP-3, His Tag (Cat. No. IG3-H5229) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-II, Avitag,Fc Tag (Cat. No. IG2-H82F9) with a linear range of 0.008-0.1 μg/mL (QC tested).
- BackgroundInsulin-like growth factor 2 (IGF-2) is also known as Somatomedin-A, IGF-II, PP9974, and is one of three protein hormonesthat share structural similarity to insulin. IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor (also called the cation-independent mannose 6-phosphate receptor), which acts as a signalling antagonist; that is, to prevent IGF2 responses. The major role of IGF2 is as a growth promoting hormone during gestation. In the process of Folliculogenesis, IGF2 is created by Theca cells to act in an autocrine manner on the theca cells themselves, and in a paracrine manner on Granulosa cells in the ovary. IGF2 promotes granulosa cell proliferation during the follicular phase of the menstrual cycle, acting alongside Follicle Stimulating Hormone (FSH). After ovulation has occurred, IGF-2 promotes progesterone secretion during the luteal phase of the menstrual cycle together with Luteinizing Hormone (LH). Thus, IGF2 acts as a Co-hormone together with both FSH and LH. IGF-2may play a key role in memory and could potentially be used to treat Alzheimer"s Disease. It is sometimes produced in excess in islet cell tumours, causing hypoglycemia. Doege-Potter syndrome is a paraneoplastic syndrome in which hypoglycemia is associated with the presence of one or more non-islet fibrous tumors in the pleural cavity. has been shown to interact with IGFBP3 and Transferrin.
- References
- (1)MacRae, Fiona , 2011, Daily Mail (London).
- (2)Balduyck B,et al., 2006, J Thorac Oncol 1 (6): 588–90.
- (3)Storch, S, et al., 2001, FEBS Lett. (Netherlands) 509 (3): 395–8.
- (4)Buckway, C K, et al., 2001, J. Clin. Endocrinol. Metab. (United States) 86 (10): 4943–50.
- (5)Twigg, S M, Baxter R C, 1998, J. Biol. Chem. (UNITED STATES) 273 (11): 6074–9.
- (6)Firth, S M; Ganeshprasad U, Baxter R C, 1998, J. Biol. Chem. (UNITED STATES) 273 (5): 2631–8.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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而国内的只有兔抗牛。
就二抗来说,国内的标记二抗质量也很不错。可以用。
特点
零耐药性,零药物残留,调控瘤胃微生物,提高饲料吸收,提高产奶量、降低体细胞、防治隐性乳房炎。
主要成分
抗牛乳房炎特异性IgY、乳酸芽孢杆菌、嗜酸乳杆菌、螯合维生素、半胱胺肽、全卵蛋白肽、活性酵母菌、螯合铁、铜、锌,抗病因子、保健因子、有益菌、生物活性复合酶、生物活性小肽等多种高档原料。
产品性状
本品呈黄褐色粉末,有特别的发酵芳香味。
功能特点
采用“三步多相联合发酵”专利技术生产,经深层发酵,离心、真空低温干燥后,辅以免疫多糖和多种科学配制而成。
1、可彻底分解霉变饲料中的弱极性霉菌毒素,可完全替代预防用抗生素;
2、可明显提高奶牛生产的各个指标,试验证明提高产奶量8-20%。延长产奶高峰15-35天,提高乳脂率0.25-0.4%,同时提高乳蛋白含量0.02-0.05%;
3、对防治奶牛乳房炎有特效,可降低乳房炎发病率60-70%,同时防治奶牛酸奶症效果突出;
4、加快犊牛瘤胃发育,可有效降低腹泻率47%,提高饲料利用率2.5%;
5、生物学稳定性高,耐挤压、耐酸碱(PH3-9)、耐高温(90℃加热30分钟活菌损失不到1%);
6、减少粪便排泄量,可降低牛舍氨臭味,减少。
应用范围:
犊牛、青年牛、高产奶牛、干乳奶牛。
使用方法:
①犊牛本品1000g拌料1000-2000斤,如发生腹泻时本品1000g拌料500斤均匀混饲;
②青年牛和干乳奶牛本品1000g拌料1000-2000斤,均匀混饲;
③高产奶牛本品1000g拌料500-1000斤,均匀混饲,如遇天气突变、转群、噪音等各种应激情况本品1000g拌料500-1000斤添加使用。
望采纳!!
中新网2月26日电据美联社报道,科学家称已经***了猴子抵抗艾滋病毒感染的机密。这是一个让人欣喜的重大发现,因为接下来的研究很可能发现一个人类预防艾滋病的新策略。
某些灵长目动物可以防止艾滋病菌在体内蔓延,这一谜团困扰科学家长达10年之久,如今终于有了答案。
美国健康研究所基础科学研究室主任称,人们根据这一发现可能研制出治疗爱滋的药物,或者是预防爱滋的疫苗。“我们将立即就15个不同方向进行研究。这将是基础研究意义重大的一年,我们取得了很多的研究成果,而且很多工作还在继续。”
此项发现是由约瑟夫.缲德劳斯克博士和他领导的哈佛大学研究人员首先公布的。约瑟夫现在波士顿达纳法伯癌症研究所工作。报告结果发表在2月27日《自然》杂志上。
艾滋病毒的发病原理是病毒先进入细胞内部,强占细胞组织,并用来不断进行自我复制。但是猴子体内有一种名为TRIM5-alpha的蛋白成分,可以阻止艾滋病毒进入健康细胞后脱落自身保护层。虽然对于病毒保护层的脱落问题还有待进一步认识,但是科学家认为这是艾滋病毒感染周期的一个重要环节。
加利福尼亚大学一位研究艾滋病的微生物专家称,人类体内也有名为TRIM5-alpha的成分,但是抵抗艾滋病毒的能力远远不如猴子体内的同类成分。
哥伦比亚大学一位生物化学家称,“由于这个报告的出台,各个大学的实验室都将就此问题进行研究。”
缲德劳斯克博士称在动物身上还可能找到治疗其它病毒的成分。“我们发现的也许只是自然预防系统中的第一个例子,通过同样的方法,我们很可能找到对抗其它病毒的成分。”
艾滋病毒是一种逆转录酶病毒,它可以把基因信息写入感染细胞当中,并且进行不断的复制。所以一旦感染,就很难根除。但是逆转录酶病毒也有弱点,它们如果一开始找不到立足点的话,就会很快消亡。所以TRIM5-alpha蛋白有助于在感染的初始阶段阻止病毒的蔓延。“我们知道艾滋病毒也可以感染猴子的细胞,但是感染一开始就被控制住或者终止了。只是我们还不清楚具体的控制时间。”
英文名称:horseradish peroxidase;HRP
定义:一种糖蛋白,由于在辣根中该酶的含量很高,故名。它以铁卟啉为辅基,在过氧化氢存在时能催化苯酚、苯胺及其取代物聚合。
确定了需要检测样品的种属,就可以选择对应的一抗
而二抗是根据一抗来选择的,比如一抗是小鼠来源的,二抗就要是抗小鼠的
1.若都为正常,那么并未有太大问题,只要定期复查就可以了。
2.如果TGAb、TMAb、甲状腺功能均高,那么就是桥本氏甲状腺炎并甲亢,需要抗甲亢治疗。
3.如果TGAb、TMAb高,甲状腺功能下降,那么就是桥本氏甲状腺炎并甲减,需要进行甲减治疗。
4.如果TGAb、TMAb高,甲状腺功能正常,那么就是桥本氏甲状腺炎,无需特殊治疗,只要定期复查甲状腺功能就可能以了,不过这种情况有可能以后演变成甲减或者甲亢。

