御神巫女IF破解版_御神巫女IF破解版下载 Ver1.1 中文更新版 ...
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DescriptionFireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening1,2.Itisaverysensitivegeneticreporterduetothelackofanyendogenousactivityinmammaliancellsortissues3,4.TheFireflyluciferaseisa62,000Daltonprotein,whichisactiveasamonomeranddoesnotrequiresubsequentprocessingforitsactivity.TheenzymecatalyzesATP-dependentD-luciferinoxidationbyoxygenintooxyluciferinwithemissionoflightcenteredon560nm(Figure1).Aswithmanyenzymes,FireflyluciferasefollowsMichaelis-Mentenkineticsand,asaresult,maximumlightoutputisnotachieveduntilthesubstrateandco-factorsarepresentinlargeexcess.Whenassayedundertheseconditions,lightemittedfromthereactionisdirectlyproportionaltothenumberofluciferaseenzymemolecules.ThisFireflyluciferaseassaykitisdesignedfordetectionandquantificationoftheFireflyluciferasereporterenzymefromculturedcellsinasimple,efficient,andlinearfashion(Figure3).Figure1.BioluminescentreactioncatalyzedbyFireflyluciferase.RenillaLuciferasehasbeenusedasareporterforstudyinggeneregulationandfunctioninvitroandinvivo5,6.Recently,RenillaLuciferasehasbeenwidelyusedinmultiplextranscriptionalreporterassaysorasanormalizingtransfectioncontrolforFireflyluciferaseassay6,7.Renillaluciferase,amonomeric36,000Daltonprotein,catalyzescoelenterazineoxidationbyoxygent
DescriptionFireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening1,2.Itisaverysensitivegeneticreporterduetothelackofanyendogenousactivityinmammaliancellsortissues3,4.TheFireflyluciferaseisa62,000Daltonprotein,whichisactiveasamonomeranddoesnotrequiresubsequentprocessingforitsactivity.TheenzymecatalyzesATP-dependentD-luciferinoxidationbyoxygenintooxyluciferinwithemissionoflightcenteredon560nm(Figure1).Aswithmanyenzymes,FireflyluciferasefollowsMichaelis-Mentenkineticsand,asaresult,maximumlightoutputisnotachieveduntilthesubstrateandco-factorsarepresentinlargeexcess.Whenassayedundertheseconditions,lightemittedfromthereactionisdirectlyproportionaltothenumberofluciferaseenzymemolecules.ThisFireflyluciferaseassaykitisdesignedfordetectionandquantificationoftheFireflyluciferasereporterenzymefromculturedcellsinasimple,efficient,andlinearfashion(Figure3).Figure1.BioluminescentreactioncatalyzedbyFireflyluciferase.RenillaLuciferasehasbeenusedasareporterforstudyinggeneregulationandfunctioninvitroandinvivo5,6.Recently,RenillaLuciferasehasbeenwidelyusedinmultiplextranscriptionalreporterassaysorasanormalizingtransfectioncontrolforFireflyluciferaseassay6,7.Renillaluciferase,amonomeric36,000Daltonprotein,catalyzescoelenterazineoxidationbyoxygent
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发布于 ： 2018-06-12
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