Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000IHC1:250
- Species:
- Rabbit
- GeneName:
- MAPK14
- MolecularReference:
- ~39kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p190-1802,RRID:AB_2492195
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr180andphospho-Tyr182ofratp38MAPK.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
ThethreeMitogen-ActivatedProteinKinases(MAPKs)areevolutionarilyconservedproteinkinasesthatcontrolavastarrayofcellularprocesses.p38MAPKisoneofthesekinasesanditisactivatedbybothinflammatorycytokinesandbystress(JohnsonandLapadat,2002;ShiandGaestel,2002).Thep38MAPKisthoughttobeparticularlyimportantindiseaseslikeasthmaandautoimmunitybutitalsoplaysimportantrolesinthestressresponseofthenervoussystem(PhilipandArmstead,2003;Yingetal.,2002).LiketheotherMAPKs,p38isactivatedbyadualspecificitykinasethatphosphorylatesThr180andTyr182(Linetal.,1995).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Perrott,K.M.,Wiley,C.D.,Desprez,P.Y.andCampisi,J.,2017.Apigeninsuppressesthesenescence-associatedsecretoryphenotypeandparacrineeffectsonbreastcancercells.GeroScience,pp.1-13.PMID:28378188
Xu,P.,Zhou,Z.,Xiong,M.,Zou,W.,Deng,X.,Ganaie,S.S.,Kleiboeker,S.,Peng,J.,Liu,K.,Wang,S.andYe,S.Q.,2017.ParvovirusB19NS1proteininducescellcyclearrestatG2-phasebyactivatingtheATR-CDC25C-CDK1pathway.PLoSpathogens,13(3),p.e1006266.PMID:28264028
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慢性淋巴细胞性甲状腺炎亦称桥本甲状腺炎,是一种自体免疫性疾病。
本病多见于中年女性,表现为甲状腺肿,起病缓慢,常在无意中发现,体积约为正常甲状腺的2~3倍,表面光滑,质地坚韧有弹性如橡皮,明显结节则少见,无压痛,与四周无粘连,可随吞咽运动活动。
若出现甲状腺机能亢进症的表现时,宜吃得清淡,吃含维生素高的新鲜蔬菜、水果及营养丰富的瘦肉、鸡肉、鸭肉、甲鱼、淡水鱼、香菇、银耳、百合、桑椹等食物。忌食碘、辣椒、羊肉、浓茶、咖啡等湿热或具有刺激性的食物。
若出现甲状腺机能减退症的表现时,除忌碘外,宜吃含维生素高的新鲜的蔬菜、水果,还宜胡桃肉、枸杞子、山药、芡实等食物。
其主要作用就是人体的防御军队,抵抗外来的病毒、细菌的侵袭。
抗体都是免疫球蛋白而免疫球蛋白不一定都是抗体。原因是抗体是由浆细胞产生,且能与相应抗原特异性结合发挥免疫功能的球蛋白。而免疫球蛋白是具有抗体活性或化学结构与抗体相似的球蛋白,如骨髓瘤患者血清中异常增高的骨髓瘤蛋白,是由浆细胞瘤产生,其结构与抗体相似,但无免疫功能。因此,免疫球蛋白可看做是化学结构上的概念,抗体则是生物学功能上的概念。

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