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Thegenecodingforp53oncoproteinislocatedonchromosome17p.Allelelossatthishromosomesitehasfrequentlybeenseeninmanytumoursincludinglung,colon,breastandbrain.Expressionofp53oncoproteinwasnotdetectedinnormalmucosa,whereasmutationresultsinadetectableexpressionofthep53protein.Itisthereforesuggestedthatimmunocytochemicaldetectionofp53proteinisanindicationformalignancy.