SyntheticpeptidewithinHumanCoRESTaa1-100.Theexactsequenceisproprietary.
Databaselink:Q9UKL0
HeLa,Molt-4andJurkatcelllysates;HumancervixcarcinomaandRatcolontissues;HeLacells.
Thisproductisarecombinantmonoclonalantibody,whichoffersseveraladvantagesincluding:-Highbatch-to-batchconsistencyandreproducibility-Improvedsensitivityandspecificity-Long-termsecurityofsupply-Animal-freeproductionFormoreinformationseehere.
OurRaBMAbtechnologyisapatentedhybridoma-basedtechnologyformakingrabbitmonoclonalantibodies.Fordetailsonourpatents,pleaserefertoRabMAbpatents.
存放说明Shippedat4°C.Storeat+4°Cshortterm(1-2weeks).Upondeliveryaliquot.Storeat-20°Clongterm.Avoidfreeze/thawcycle.
存储溶液Preservative:0.01%Sodiumazide
Constituents:59%PBS,40%Glycerol,0.05%BSA
Theapplicationnotesincluderecommendedstartingdilutions;optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
Nuclearreceptorcoregulator1antibodyNuclearreceptorsubfamily2groupBmember2antibodyProteinCoRESTantibodyRCORantibodyRcor1antibodyRCOR1_HUMANantibodyRESTcorepressor1antibodyRetinoicacidreceptorRXR-betaantibodyRetinoidXreceptorbetaantibodyRxrbantibodyseeall
Alllanes:Anti-CoRESTantibody[EPR13825](ab183711)at1/10000dilution
Lane1:HeLacelllysate
Lane2:Molt-4celllysate
Lane3:Jurkatcelllysate
Lysates/proteinsat20µgperlane.
Secondary
Alllanes:GoatAnti-RabbitIgG,(H+L),Peroxidaseconjugateat1/1000dilution
Predictedbandsize:53kDa
Observedbandsize:66kDawhyistheactualbandsizedifferentfromthepredicted?
Immunohistochemicalanalysisofparaffin-embeddedHumancervixcarcinomatissuelabelingCoRESTwithab183711at1/50dilutionfollowedbypredilutedHRPPolymerforRabbitIgG.CounterstainedwithHematoxylin.
PerformheatmediatedantigenretrievalwithEDTAbufferpH9beforecommencingwithIHCstainingprotocol.
Immunohistochemicalanalysisofparaffin-embeddedRatcolontissuelabelingCoRESTwithab183711at1/50dilutionfollowedbypredilutedHRPPolymerforRabbitIgG.CounterstainedwithHematoxylin
PerformheatmediatedantigenretrievalwithEDTAbufferpH9beforecommencingwithIHCstainingprotocol.
Immunofluorescentanalysisofacetone-fixedHeLacellslabelingCoRESTwithab183711at1/100dilution,followedbyGoatantirabbitIgG(AlexaFluor488)secondaryantibodyat1/200dilution(green).CounterstainedwithDapi(blue).
TodownloadaCertificateofCompliance,pleaseenteryourLotnumberbelow:
Lotnumber发表研究结果有使用ab183711？请让我们知道，以便我们可以引用本数据表中的参考文章。
ab183711被引用在2文献中.
MehtaSLetal.LongNoncodingRNAFosDTPromotesIschemicBrainInjurybyInteractingwithREST-AssociatedChromatin-ModifyingProteins.JNeurosci35:16443-9(2015).PubMed:26674869ChanFCetal.AnRCOR1loss-associatedgeneexpressionsignatureidentifiesaprognosticallysignificantDLBCLsubgroup.Blood125:959-66(2015).HumanPubMed:25395426SampleRatTissuelysate-nuclear(Hippocampus,liverandMCF7wholecellhpmogenates)
BlockingstepMilkasblockingagentfor1hour(s)and0minute(s)Concentration:5%Temperature:25C
Pleasenote:AllproductsareFORRESEARCHUSEONLY.NOTFORUSEINDIAGNOSTICPROCEDURES
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