
The monoclonal antibody to mouse IL-4 (clone 11B11) has shown to be suitable for intracellular staining (Assenmacher M. et al. 1998), ELISA (Carson R.T. et al. 1999) and Western Blot. The 11B11 antibody can also successfully be used in immunohistochemistry and neutralization assays (Chaudhri G. et al. 2004 and Hammond K.J. et al. 1998).


References list
- Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.
- Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay.
- Polarized type 1 cytokine response and cell-mediated immunity determine genetic resistance to mousepox.
- Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
- An immunoadhesin incorporating the molecule OX-2 is a potent immunosuppressant that prolongs allo- and xenograft survival.
- Administration of anti-IL-4 monoclonal antibody 11B11 increases the resistance of mice to Listeria monocytogenes infection.
- alpha/beta-T cell receptor (TCR)+CD4-CD8- (NKT) thymocytes prevent insulin-dependent diabetes mellitus in nonobese diabetic (NOD)/Lt mice by the influence of interleukin (IL)-4 and/or IL-10.
- Involvement of Th2 cytokines in the mouse model of flutamide-induced acute liver injury.
- IL-4 mediates dicloxacillin-induced liver injury in mice.
- Th2 cytokine-mediated methimazole-induced acute liver injury in mice.
- Cholera toxin B subunit binding to an antigen-presenting cell directly co-stimulates cytokine production from a T cell clone.
- Isolation of monoclonal antibodies specific for IL-4, IL-5, IL-6, and a new Th2-specific cytokine (IL-10), cytokine synthesis inhibitory factor, by using a solid phase radioimmunoadsorbent assay.
- Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1.
- B-cell stimulatory factor-1/interleukin 4.
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请问各位大神,需要做人巨噬细胞的F4/80,CD11b,CD163标记物,用流式,抗体哪家比较好呢,请推荐,谢谢!
比如,你使用了小鼠IgG1, PE 标记的特异性抗体,那就选用小鼠IgG1, PE标记的非特异性抗体。
下面这个图,是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本,黄色是同型对照,红色是特异性抗体染色。由于细胞对抗体的非特异性结合,一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候,要根据同型对照,而不是未染色的细胞。
请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书

