
The rat anti-human IL-5 monoclonal antibody (clone TRFK5) was first described in 1988. The TRFK5 antibody recognizes and neutralizes both human and mouse IL-5 (Schumacher J.H. et al. 1988). The antibody has been descriped as useful in a sandwich ELISA (Schumacher J.H. et al. 1988) and the ELISPOT assay (Taguchi T. et al. 1990). The TRFK5 antibody has also been reported for use in intracellular staining (Belkaid Y. et al. 1998), immunohistochemical staining (Fan W.Y. et al. 2001) and Western Blot (Zhang J. et al. 1999).


References list
- Development of a natural model of cutaneous leishmaniasis: powerful effects of vector saliva and saliva preexposure on the long-term outcome of Leishmania major infection in the mouse ear dermis.
- Effects of dietary restriction on spontaneous dermatitis in NC/Nga mice.
- The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent.
- Detection of individual mouse splenic T cells producing IFN-gamma and IL-5 using the enzyme-linked immunospot (ELISPOT) assay.
- Mapping and characterization of the epitope(s) of Sch 55700, a humanized mAb, that inhibits human IL-5.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
有哪位比较明白的能给我提点建议么,这两天真是晕到家了。谢谢!

