
α-tubulin Mouse Monoclonal AntibodyPDF
Catalog Number:
E12-048Amount:
100μg/100μlClone Number:
8F11Background:
Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulinForm of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 year. Do not aliquot the antibody.Specificity/Sensitivity:
The α-tubulin antibody can detects endogenousα-tubulin proteinReactivity:
H, R, MApplications:
WB: 1:5,000-10,000 IP:1:200 IF: 1:100-200Price:
150$Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with E12-048 diluted at 1:5,000.1、Input: Mouse Brain Tissue Lysate2、IP product: IP dilute 1:200Western blot analysis: primary antibody : E12-048 1:5,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000
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多抗虽然可能可以产生更强的信号,但是特异性差,染色后荧光强度和抗原水平不是线性关系,不同批次差异很大。所以流式检测不使用多抗。
如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。

