
Product Name | HCV NS3/4A protease genotype 1b, recombinant |
Size | 10 µg |
Catalog # | AS-61017-10 |
US$ | $379 |
The NS3 protease of hepatitis C virus (HCV) is responsible for the cleavage at the NS3/NS4A, NS4A/NS4B, NS4B/NS5A, and NS5A/NS5B sites of the nonstructural protein. It is essential for viral replication and the formation of infectious viral particles, and thus has been considered as one of the most attractive targets for anti-HCV therapy. Recombinant HCV NS3/4A protease is highly active and can be used with SensoLyte® 490 or 520 HCV Protease Assay Kits (Cat# 71126, 71145) to screen for anti-HCV protease drugs. | |
Detailed Information | ![]() ![]() ![]() |
Storage | Store at -80°C. Avoid multiple thaw-freeze cycles. |
References | 1. R. Bartenschlager, R. et al. J.Virol. 67, 3835-3844 (1993).2. Eckart, MR. et al. Biochem.Biophys.Res.Commun. 192, 399-406 (1993).3. Landro, JA. et al. Biochemistry 36, 9340-9348 (1997).4. Kim, JL. et al. Cell 87, 343-355 (1996).5. Love, RA. et al. Cell 87, 331-342 (1996). |
Product Citations | Ma, C. et al. (2009). HCV Protease Inhibitory, Cytotoxic and Apoptosis-Inducing Effects of Oleanolic Acid Derivatives. J Pharm Pharmaceut Sci 12, 243.Phuong, DT. et al. (2009). Inhibitory effects of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. Phytother Res 23, 582.of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. Phytother. Res. 23, 582.Yu, X. (2009). Development of a cell-based hepatitis C virus (HCV) infection FRET assay for high throughput antiviral compound screening. Antimicrob Agents Chemo doi:10.1128/AAC.00495-09.Ma, C. et al. (2007). Triterpenes from Cynomorium songariciumanalysis of HCV protease inhibitory activity, quantification, and content change under the influence of heating. J Nat Med 63, 9. |
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如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
有哪位比较明白的能给我提点建议么,这两天真是晕到家了。谢谢!

