

This product is freeze dried. All water molecules have been removed.

This antibody is shipped with its antigen FREE of charge!
- Peptide (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1 (Accession O00299). Intracellular, C-terminus.
- Western blot analysis of human colorectal adenocarcinoma HT-29 (lanes 1 and 5), T-84 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates:1-4. Anti-CLIC1 Antibody (#ACL-021), (1:200).
5-8. Anti-CLIC1 Antibody, preincubated with the negative control antigen.
- Expression of CLIC1 in human colorectal adenocarcinoma HT-29 cellsImmunocytochemical staining of fixed and permeabilized human colorectal adenocarcinoma HT-29 cells. A. Cells were stained with Anti-CLIC1 Antibody (#ACL-021), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
- 1. Edwards, J.C. and Kahl, C.R. (2010) FEBS Lett. 584, 2102.
- 2. Ashley, R.H. (2003) Mol. Membr. Biol. 20, 1.
- 3. Cromer, B.A. et al. (2002) Eur. Biophys. J. 31, 356.
- 4. Ulmasov, B. et al. (2007) BMC Cell. Biol. 8, 8.
- 5. Jung, J.J. and Kitajewski, J. (2010) J. Angiogenes. Res. 2, 23.
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Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3.
The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associated with the plasma membrane) via their single transmembrane domain1.
When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend on whether CLICs are membrane bound or cytosolic1.
CLIC1, which was first identified in nuclear membranes, is detected in the epithelia of airways, the gall bladder, pancreas, stomach, small intestine and colon4. CLIC1 was also found to be expressed in the endothelium where it plays a role in cell growth and migration5.
Anti-CLIC1 Antibody (#ACL-021) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CLIC1 from mouse, rat, and human samples.
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有哪位比较明白的能给我提点建议么,这两天真是晕到家了。谢谢!
高保真生物技术有限公司~~实验整体外包服务商~~~
能用的条件:1.分子的小鼠和人差异小,2.小鼠细胞本身不含有鼠抗体的结合受体;
不能用的条件:1.分子种属间差异大,2.采用的小鼠细胞株表面有鼠抗体的结合受体。
推荐尽量采用抗鼠的抗体,义翘神州兔单抗技术做出来的FCM抗体不少,可以到官网搜一下看看。
流式细胞仪能检测多少个通道
需要同时检测检测多少个指标
厂家的抗体有多少种荧光标记
如果流式细胞仪的通道越多,那么同一份样本能同时做的表面/胞内标志就越多
A、 如何根据流式细胞仪搭配流式抗体
1) BD流式细胞仪
BD流式试剂选择及应用的相关问答与简介
流式细胞仪能检测多少个通道是由有多少根激光决定的以及每根激光的功能决定的,所以首先需要注意两点:
流式细胞仪有哪些激光。比如标配的FACSCalibur只有一根488nm的激光,能做FL1、FL2、FL3三个荧光通道/三个颜色,而选配的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光,可以检测FL1-FL4四个通道/4个颜色。
不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的,比如Calibur的FL3(第3通道)能检测PE-Texas Red, PE-Cy5, PerCP, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-Texas Red, PE-Cy5, PerCP。 Calibur第3通道能检测的PerCP-Cy5.5在FACSAria上是第4通道检测。Calibur和LSR都能检测4个颜色,但是第4个通道检测荧光素是不一样的
比如,你使用了小鼠IgG1, PE 标记的特异性抗体,那就选用小鼠IgG1, PE标记的非特异性抗体。
下面这个图,是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本,黄色是同型对照,红色是特异性抗体染色。由于细胞对抗体的非特异性结合,一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候,要根据同型对照,而不是未染色的细胞。
如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊

