

This product is freeze dried. All water molecules have been removed.

This antibody is shipped with its antigen FREE of charge!
- Peptide (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1 (Accession O00299). Intracellular, C-terminus.
- Western blot analysis of human colorectal adenocarcinoma HT-29 (lanes 1 and 5), T-84 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates:1-4. Anti-CLIC1 Antibody (#ACL-021), (1:200).
5-8. Anti-CLIC1 Antibody, preincubated with the negative control antigen.
- Expression of CLIC1 in human colorectal adenocarcinoma HT-29 cellsImmunocytochemical staining of fixed and permeabilized human colorectal adenocarcinoma HT-29 cells. A. Cells were stained with Anti-CLIC1 Antibody (#ACL-021), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
- 1. Edwards, J.C. and Kahl, C.R. (2010) FEBS Lett. 584, 2102.
- 2. Ashley, R.H. (2003) Mol. Membr. Biol. 20, 1.
- 3. Cromer, B.A. et al. (2002) Eur. Biophys. J. 31, 356.
- 4. Ulmasov, B. et al. (2007) BMC Cell. Biol. 8, 8.
- 5. Jung, J.J. and Kitajewski, J. (2010) J. Angiogenes. Res. 2, 23.
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Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3.
The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associated with the plasma membrane) via their single transmembrane domain1.
When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend on whether CLICs are membrane bound or cytosolic1.
CLIC1, which was first identified in nuclear membranes, is detected in the epithelia of airways, the gall bladder, pancreas, stomach, small intestine and colon4. CLIC1 was also found to be expressed in the endothelium where it plays a role in cell growth and migration5.
Anti-CLIC1 Antibody (#ACL-021) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CLIC1 from mouse, rat, and human samples.
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如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
请问各位大神,需要做人巨噬细胞的F4/80,CD11b,CD163标记物,用流式,抗体哪家比较好呢,请推荐,谢谢!
比如,你使用了小鼠IgG1, PE 标记的特异性抗体,那就选用小鼠IgG1, PE标记的非特异性抗体。
下面这个图,是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本,黄色是同型对照,红色是特异性抗体染色。由于细胞对抗体的非特异性结合,一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候,要根据同型对照,而不是未染色的细胞。
高保真生物技术有限公司~~实验整体外包服务商~~~

