
- Species ReactivityHuman
- SpecificityDetects recombinant human PILR-betain direct ELISAs and Western blots. In Western blots, approximately 10% cross reactivity with recombinant human PILR-alphais observed and less than 5% cross-reactivity with recombinant mouse (rm) PILR-beta, rmPILR-alpha, and rmPILR-L is observed.
- SourcePolyclonal Sheep IgG
- PurificationAntigen Affinity-purified
- ImmunogenMouse myeloma cell line NS0-derived recombinant human PILR-betaisoform 1
Gln20-Ala189
Accession # Q9UKJ0 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Western Blot0.1 µg/mLRecombinant Human PILR-beta
- Flow Cytometry2.5 µg/106 cellsSee below
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:Paired-Ig-likeType 2 Receptor beta
- Entrez Gene IDs:29990 (Human); 170741 (Mouse)
- Alternate Names:activating receptor PILRbeta; Activating receptor PILR-beta; Cell surface receptor FDFACT; cell surface receptor FDFACT1; cell surface receptor FDFACT2; FDFACT; FDFACT1; FDFACT2; paired immunoglobin-like receptor beta; paired immunoglobin-like type 2 receptor beta; paired immunoglobulin-like receptor beta; paired immunoglobulin-like type 2 receptor beta; PILRB; PILRbeta; PILR-beta
Background:
Paired immunoglobulin-like, type 2 receptor beta (PILR-beta ) is a type I transmembrane (TM) glycoprotein belonging to the Ig superfamily. It is the activating counterpart to the ITIM-bearing PILR-alphainhibitory receptor. PILR-betais expressed in a wide variety of tissues including hematopoietic cells. Mature human PILR-betais a 208 amino acid (aa) protein with one V-type Ig-like extracellular domain, a truncated cytoplasmic tail, and positively-charged residues in its TM domain that interacts with ITAM‑bearing adaptor molecules. Within the V-type Ig-like region in their ECD, human PILR-betaand PILR-alphashare a 92% aa sequence identity. The aa sequence of mouse PILR-betaECD is only 43% identical to that of the human protein.
ebiomall.com






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如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
有哪位比较明白的能给我提点建议么,这两天真是晕到家了。谢谢!


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