- Species ReactivityHuman
- SpecificityDetects human Uteroglobin/SCGB1A1 in direct ELISAs.
- SourcePolyclonal Sheep IgG
- PurificationAntigen Affinity-purified
- ImmunogenE. coli-derived recombinant human Uteroglobin/SCGB1A1
Glu22-Asn91
Accession # NP_003348 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- Endotoxin Level<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">
- LabelUnconjugated
- Immunohistochemistry5-15 µg/mLSee below
- NeutralizationMeasured by its ability to neutralize Uteroglobin-mediated adhesion of A549 human lung carcinoma cells. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 5 µg/mL Recombinant Human Uteroglobin.
- ReconstitutionSterile PBS to a final concentration of 0.2 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.
- Singh, G. et al. (1988) Biochim. Biophys. Acta 950:329.
- Mantile, G. et al. (1993) J. Biol. Chem. 268:20343.
- Wang, S-Z. et al. (2003) J. Immunol. 171:1051.
- Umland, T.C. et al. (1994) Nat. Struct. Biol. 1:538.
- Mandal, A.K. et al. (2004) J. Exp. Med. 199:1317.
- Watson, T.M. et al. (2001) Am. J. Physiol. Lung Cell Mol. Physiol. 281:L1523.
- Yang, Y. et al. (2004) J. Biol. Chem. 279:29336.
- Ray, R. et al. (2006) FEBS Lett. 580:6022.
- Martin, A.C. et al. (2006) Am. J. Respir. Crit. Care Med. 173:617.
- Antico, G. et al. (2006) J. Cell Physiol. 207:553.
- Zheng, F. et al. (1999) Nat. Med. 5:1018.
- Zhang, Z. et al. (2006) Gene 369:66.
- Entrez Gene IDs:7356 (Human); 22287 (Mouse); 25575 (Rat)
- Alternate Names:Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin
Background:
Uteroglobin, also called Clara cell secretory, phospholipid binding, 10 kDa or 16 kDa protein (CCSP, CCPBP, CC10 or CC16, respectively) is a small, non‑glycosylated secreted protein of the secretoglobin superfamily, (designated 1A, member 1) (1‑3). Its name is derived from its very high expression in the pre‑implantation uterus. It is produced by the non-ciliated, non-mucous secretory cells that predominate in lung bronchioles (Clara cells), and other non-ciliated epithelia that communicate with the external environment (1‑3). Expression is induced by steroid hormones such as estrogen, and enhanced by the non-steroid hormone prolactin (1). Uteroglobin is found in blood, urine and other body fluids (1). Human Uteroglobin cDNA encodes a 21 amino acid (aa) signal sequence and a 70 aa mature protein. It shares 53-56% aa identity with mouse, rat, bovine, canine, equine or rabbit Uteroglobin, and is active in mice (4). The mature protein forms a disulfide-linked head-to-tail homodimer of 16 kDa (2, 5). This homodimer is thought to form a binding pocket that binds hydrophobic ligands such as phospholipids, progesterone and retinols (5). Sequestering of prostaglandins and leukotrienes is anti-inflammatory, while sequestering of carcinogens such as polychlorinated bisphenols is anti-tumorigenic (6‑8). Other immunoregulatory activities of Uteroglobin include cell migration inhibition (by binding the chemotaxis-related formyl peptide receptor FPR2 on dendritic cells), and the inhibition of T cell differentiation to a Th2 phenotype (9). A single nucleotide polymorphism of Uteroglobin, A38G, confers increased risk of asthma (10). Transglutaminase can crosslink Uteroglobin, either to itself or to other proteins such as the adhesion molecule fibronectin (3, 11). Binding of fibronectin to Uteroglobin in the kidney is thought to protect against nephropathy, while binding of the lipocalin-1 receptor has been reported to suppress cancer cell motility and invasion (12, 13).
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如果一个抗体可以用来“做western blot，免疫荧光，免疫共沉淀,ELISA”，我估计着是HRP或碱性磷酸酶或生物素之类标记的，然后需要再加荧光标记的二抗，这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光（直接染色），效果还不错，但是反过来就没试过。
如果一个抗体可以用来“做western blot，免疫荧光，免疫共沉淀,ELISA”，我估计着是HRP或碱性磷酸酶或生物素之类标记的，然后需要再加荧光标记的二抗，这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光（直接染色），效果还不错，但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
请问一下做流式需要的抗体那种比较好，有人说Biolegend，eBioscience，santa，BD。求各位大神指教
有哪位比较明白的能给我提点建议么，这两天真是晕到家了。谢谢！
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