Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
Neurotrophin; Abrineurin
- Product No.MAA011Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)n/a
- Ig IsotypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogenn/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.If the antibody is used in flow cytometry, please check FCM antibodies.
- Downloadn/a
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 87 For more details, please contact local distributors!US$ 203 For more details, please contact local distributors!US$ 290 For more details, please contact local distributors!US$ 725 For more details, please contact local distributors!US$ 2900 For more details, please contact local distributors!
SPECIFITY of the Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
The antibody isa mouse monoclonal antibody raised against BDNF. It has been selected for its ability to recognize BDNF in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
Western blotting: 0.2-2µg/mL;1:500-5000 Immunohistochemistry: 5-20µg/mL;1:50-200 Immunocytochemistry: 5-20µg/mL;1:50-200 Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized ServiceProtein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitLong-arm Biotin Labeling KitReal Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA011Hu01 | Recombinant Brain Derived Neurotrophic Factor (BDNF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA011Hu02 | Recombinant Brain Derived Neurotrophic Factor (BDNF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA011Hu61 | Active Brain Derived Neurotrophic Factor (BDNF) | Cell culture; Activity Assays. |
| EPA011Hu61 | Eukaryotic Brain Derived Neurotrophic Factor (BDNF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA011Hu01 | Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IP. |
| PAA011Hu06 | Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IP. |
| LAA011Hu71 | Biotin-Linked Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC. |
| LAA011Hu81 | FITC-Linked Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IF. |
| MAA011Hu22 | Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF) | WB; IHC; ICC; IP. |
| SEA011Hu | ELISA Kit for Brain Derived Neurotrophic Factor (BDNF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA011Hu | CLIA Kit for Brain Derived Neurotrophic Factor (BDNF) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA011Hu01 | ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF) | Main materials for "Do It (ELISA Kit) Yourself". |
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希望得到前辈们的回答。非常感谢!
鼠源性抗体应用于人体会产生抗鼠抗体反应(HAMA )等,妨碍其在临床上的应用,所以要制备人鼠杂交和完全人源化的抗体,减少抗体中的鼠源成分,尽量保留原有抗体的特异性.
单克隆抗体这项新技术从根本上解决了在抗体制备中长期存在的特异性和可重复性问题,可用于探讨: ①蛋白质的精细结构;②淋巴细胞亚群的表面新抗原;③组织相容性抗原;④激素和药物的放射免疫(或酶免疫)分析;⑤肿瘤的定位和分类;⑥纯化微生物和寄生虫抗原;⑦免疫治疗和与药物结合的免疫-化学疗法 (“导弹”疗法,利用单克隆抗体与靶细胞特异性结合,将药物带至病灶部位.。
因此,单克隆抗体可直接用于人类疾病的诊断、预防、治疗以及免疫机制的研究,为人类恶性肿瘤的免疫诊断与免疫治疗开辟了广阔前景。
(1)杂交瘤可以在体外“永久”地存活并传代,只要不发生细胞株的基因突变,就可以不断地生产高特异性、高均一性的抗体。
(2)可以用相对不纯的抗原,获得大量高度特异的、均一的抗体。
(3)由于可能得到“无限量”的均一性抗体,所以适用于以标记抗体为特点的免疫学分析方法,如IRMA和ELISA等。
(4)由于单克隆抗体的高特异性和单一生物学功能,可用于体内的放射免疫显像和免疫导向治疗。
2.单克隆抗体的局限性
(1)单克隆抗体固有的亲和性和局限的生物活性限制了它的应用范围。由于单克隆抗体不能进行沉淀和凝集反应,所以很多检测方法不能用单克隆抗体完成。
(2)单克隆抗体的反应强度不如多克隆抗体。
(3)制备技术复杂,而且费时费工,所以单克隆抗体的价格也较高。向左转|向右转
一、前言
1975年,Kohler和Milstein发现将小鼠骨髓瘤细胞和绵羊红细胞免疫的小鼠脾细胞进行融合,形成的杂交细胞既可产生抗体,又可无限增殖,从而创立了单克隆抗体杂交瘤技术。这一技术上的突破不仅为医学与生物学基础研究开创了新纪元,也为临床疾病的诊、防、治提供了新的工具。
制备单克隆抗体包括动物免疫、细胞融合、选择杂交瘤、检测抗体、杂交瘤细胞的克隆化、冻存以及单克隆抗体的大量生产,要经过几个月的一系列实验步骤。
虽然单抗技术已经非常成熟,但是由于其经济价值,仍然有很多人在从事这项研究,而且其中也会遇到很多这样那样大问题。我本人就是其中一个,由于是第一次做单抗,所以过程中遇到了很多困难,终于在前几天得到了几株阳性克隆。
鉴于此,我将单克隆抗体制备的整个过程贴出来,同时搜索了园子里面一些战友的求助帖以及一些经典的应助帖,希望能对将要或是正在从事这项研究的战友们有些帮助。
由人源化和全人源抗体制备的人源化和全人源抗体药物因其具有高亲和力、高特异性、毒副作用小的特点,克服了动物源抗体及嵌合抗体的各种缺点,已经成为了治疗性抗体药物发展的必然趋势。
阿达木单抗作为生物单抗药物,在疗效和技术门槛方面难以挑战;另外,越来越多的医生在治疗类风湿时首选皮下注射给药的剂型,不需要注射过程和费用,虽然与依那西普的对比临床试验还未出来,但是人们普遍认为阿达木单抗的疗效更好。向左转|向右转
向左转|向右转
首先是一个抗体药物的releaselotstest:
?ProteinConcentration----------------------70.9mg/mL[63.0-77.0]
?Potency----------------------100%[80-125%]
?ID(immunoassay)----------------------Pass
?SE-HLC-----------------------MP:99.8%,HMW:0.2%,LMW:0.0%[MP≥99%]
?CE-SDS,reduced----------------------HC+LC:98.9%,HC:66.8%LC:32.1%[LC+HC≥98%]
?CEX-HPLC-----------------------MP:89.6%,Acidic:2.7%,Basic:7.7%[≥80%]
?CEX-HPLCID-----------------------Pass
?Osmolality-----------------------314mOsm/kg[300±50]
?pH----------------------5.2at24.6°C[5.0–5.4]
?Appearance-----------------------Report
?Volume-----------------------1.2mL(average1.1525mL,n=5)[≥1mL]
?Sub-visIBLeParticulate----------------------29particles/container≥10μm[nomorethan6000]
3particles/container≥25μm[nomorethan600]
?Sterility--------------------------------------Pass
?BacterialEndotoxin-----------------------<0.2EU/mL[≤5.0]
?CHOPELISA-----------------------4.4ppm
?Protein-AELISA-----------------------<0.5ppm
?DNAQPCR-----------------------<1pg/mg
以上各项指标基本是反映了对抗体药物最终产品的质量要求,用到的一些方法也比较的明了,可以作为抗体药物开发的参考。
版主zhulikou431留言:
加分鼓励有价值的话题。
大家好,最近在做单抗,里面有些问题一直想不通,我想请大神帮忙一下,想问一下饲养细胞加少了是大概多少,我一直6周龄的balb/C,刚拿回来就取饲养细胞了,然后取了3毫升,注射到60毫升的HAT中铺了六块板子,这个量多么,么有细胞计数,至今细胞计数不是很会,去想问你们第二天去看板子是时候,底下会有雾一样的细胞么,细胞多的地方你们的液体上清会有点变色么,要一点不变色的是好的,还是细胞上清稍微变色是正常的呢谢谢大神指导


