BackgroundThe reactivity of the antiserum is restricted to free and bound secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining. To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.

SourceHighly purified secretory component isolated from human milk.

ProductPurified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.

Product Form: TRITC

Formulation: Purified monoclonal mouse IgG1 kappaconjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

SpecificityTetramethylrhodamine isothiocyanate-conjugated purified monoclonal Mouse antibody to Human secretory component, free and bound.

Species Reactivity: The antiSerum does not react with any other component of the Human milk or plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

ApplicationsELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C.Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day.

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession number: P01833