| Isotype | IgG |
| Product Type | Antigen Immunoaffinity Purified Polyclonal |
| Units | 100 µg |
| Host | Rabbit |
| Species reactivity | Human |
| Application | ELISAImmunohistochemistryWestern Blot Loading ControlWestern Blotting |
BackgroundGAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots. Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
Source
Immunogen: Synthetic peptide derived from the human GAPDH alpha protein.
Product
Product Form: Affinity Purified
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Antigen Immunoaffiinity Purification
Concentration: See vial for concentration
ApplicationsAntibody can be used for Western Blotting (1:400 dilution) and immunohistochemistry (2-10 µg/ml).Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Human liver tissue, human testis
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
References1. Nakajima, H., et al. "GAPDH aggregate formation participates in oxidative stress-induced cell death." J. Biol. Chem. 2009:Epub2. Nahlik, K.W., et al. "Modulation of GAPDH expression and cellular localization after vaccinia virus infection of human adherent monocytes." Acta Biochim. Pol. 2003: 50(3); 667-676.
Protein Reference(s)
Database Name: UniProt
Accession number: P04406
Species Accession: Human
Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf
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制备抗体都需要进行纯化处理
多抗，稀释度更大，特异性相对较差，容易出现多条带。
兔的单克隆抗体和鼠的单克隆抗体在使用上不会有什么区别。
用来很多抗体，许多时候觉得单抗多抗也未必是理论上那样的。单抗做不好的也有，多抗条带唯一且清晰的也有。
而且很多蛋白的抗体未必有那么多的选择。
1.单克隆抗体的优点：
（1）杂交瘤可以在体外“永久”地存活并传代,只要不发生细胞株的基因突变,就可以不断的生产高特异性、高均一性的抗体.
（2）可以用相对不纯的抗原,获得大量高度特异的、均一的抗体.
（3）由于可能得到“无限量”的均一性抗体,所以适用于以标记抗体为特点的免疫学分析方法,如IRMA和ELISA等.
（4）由于单克隆抗体的高特异性和单一生物学功能,可用于体内的放射免疫显像和免疫导向治疗.
2.单克隆抗体的局限性：
(1）单克隆抗体固定的亲和性和局限的生物活性限制了它的应用范围.由于单克隆抗体不能进行沉淀和凝集反应,所以很多检测方法不能用单克隆抗体完成.
(2)单克隆抗体的反应强度不如多克隆抗体.
(3）制备技术复杂,而且费时费工,所以单克隆抗体的价格也较高.
说得简单点就是，细胞核是原封不动的，没有重组过，而细胞核外的东西是很多别的细胞中提取出来的并组合起来的。这样的混合体淋巴细胞产生的抗体具有很强的嵌合能力，针对性比一般的抗体强，但是它的来源没变，所以称之为混合单克隆抗体
