| Isotype | IgG |
| Product Type | Antigen Immunoaffinity Purified Polyclonal |
| Units | 100 µg |
| Host | Rabbit |
| Species reactivity | Human |
| Application | ELISAImmunohistochemistryWestern Blot Loading ControlWestern Blotting |
BackgroundGAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots. Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
Source
Immunogen: Synthetic peptide derived from the human GAPDH alpha protein.
Product
Product Form: Affinity Purified
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Antigen Immunoaffiinity Purification
Concentration: See vial for concentration
ApplicationsAntibody can be used for Western Blotting (1:400 dilution) and immunohistochemistry (2-10 µg/ml).Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Human liver tissue, human testis
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
References1. Nakajima, H., et al. "GAPDH aggregate formation participates in oxidative stress-induced cell death." J. Biol. Chem. 2009:Epub2. Nahlik, K.W., et al. "Modulation of GAPDH expression and cellular localization after vaccinia virus infection of human adherent monocytes." Acta Biochim. Pol. 2003: 50(3); 667-676.
Protein Reference(s)
Database Name: UniProt
Accession number: P04406
Species Accession: Human
Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf
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一般来说多克隆的阳性率高一些，但出现假阳性的比例也高一些。
制备抗体都需要进行纯化处理
抗体的特异性鉴定 抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高，它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线，计算各自的结合率，求出各自在 IC50时的浓度，并按下列公式计算交叉反应率。 如果所用抗原浓度IC50浓度为pg/管，而一些近似抗原物质的IC50浓度几乎是无穷大时，表示 这一抗血清与其他抗原物质的交叉反应率近似为 0，即该血清的特异性较好。
抗体的亲和力 是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小、抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键、疏水键、侧链相反电荷基因的库仑力、范德华力和空间斥力。亲和力常以亲和常数K表示，K的单位是L/mol，通常K的范围在 108 ～1010 /mol，也有多达 1014 /mol。抗体亲和力的测定对抗体的筛选，确定抗体的用途，验证抗体的均一性等均有重要意义。向左转|向右转
又由于自然存在的抗原大都存在多个抗原表位,会刺激机体产生多种针对同一抗原的不同抗原表位相应的不同抗体.
