FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5; Neutrophil-activating peptide ENA-78
- Product No.LAA860Hu81
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourceAntibody labeling
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelFITC
- Original Antibodyn/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IF.If the antibody is used in flow cytometry, please check FCM antibodies.
- Downloadn/a
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 95 For more details, please contact local distributors!US$ 221 For more details, please contact local distributors!US$ 316 For more details, please contact local distributors!US$ 790 For more details, please contact local distributors!US$ 3160 For more details, please contact local distributors!
SPECIFITY of the FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
The antibody isa rabbit polyclonal antibody raised against ENA78. It has been selected for its ability to recognize ENA78 in immunohistochemical staining and western blotting.
USAGE of the FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
Western blotting: 0.2-2µg/mL;1:250-2500 Immunohistochemistry: 5-20µg/mL;1:25-100 Immunocytochemistry: 5-20µg/mL;1:25-100 Optimal working dilutions must be determined by end user.
STORAGE of the of the FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Protein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitReal Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA860Hu01 | Active Epithelial Neutrophil Activating Peptide 78 (ENA78) | Cell culture; Activity Assays. |
| RPA860Hu02 | Recombinant Epithelial Neutrophil Activating Peptide 78 (ENA78) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA860Hu03 | Recombinant Epithelial Neutrophil Activating Peptide 78 (ENA78) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA860Hu01 | Recombinant Epithelial Neutrophil Activating Peptide 78 (ENA78) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA860Hu02 | Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IP. |
| PAA860Hu03 | Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IP. |
| PAA860Hu01 | Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IP. |
| LAA860Hu81 | FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IF. |
| LAA860Hu71 | Biotin-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC. |
| MAA860Hu22 | Monoclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IP. |
| SEA860Hu | ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA860Hu | CLIA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA860Hu01 | ELISA Kit DIY Materials for Epithelial Neutrophil Activating Peptide 78 (ENA78) | Main materials for "Do It (ELISA Kit) Yourself". |
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1取组织
(1) 灌注固定:暴露心脏,将注射针头插入左心室,剪开右心耳,快速灌注PBS,待右心耳处流出澄清液体,换4%多聚甲醛灌注到大鼠四肢强直为止,快速取脑和肾。
(2) 固定:将组织置于4%多聚甲醛中固定15小时
(3) 脱水:将组织放入30% 蔗糖溶液中直至下沉
(4) 切片:脑组织冰冻切片20微米,37度烘干2h以上;
(5)将切片放置-80度保存,随用随取。
2免疫单标记
(1)做抗原修复(0.4g柠檬酸、3g柠檬酸钠、1000ml水配制成溶液,放到水浴箱中90~94℃保存15min),冷却后, 用1*PBS反复洗标本10min,吸去PBS,同时向标本加渗透液(配0.4%trition液,然后将BSA加入到配好的trition液中,配成1%浓度)100ul,常温下湿盒反应1h。
(2) 吸去渗透液,同时向标本加block液(5%封闭用正常山羊血清)100ul,常温下湿盒反应5h。
(3) 吸去block液,同时向标本加入1:50稀释的羊抗鼠一抗100ul,37度湿盒反应过夜。
(4)吸去一抗,并回收,用1*PBS洗液反复洗15min。
(5)快速从冰箱中取出驴抗羊的二抗,并立即用锡纸包裹,注入到一个新的以锡纸包裹的tube管中并用PBS以1:200稀释。
(6)进入暗室,吸去PBS,同时加入二抗100ul,37度湿盒反应2h,封片。
(7)放置到荧光显微镜下观察。
其次,察看次目的蛋白的存在形式,有没有多聚体形式及变构形式;
最后,查看多家抗体公司的DATA,看看别人的WB做出来的条带的位置。
根据你说的,特异识别多个组织中的同样大小的条带,我觉得很可能就是你的目的蛋白。
不过多克隆抗体因为有多个结合位点,事实上的特异性并不如单克隆抗体,所以一般Western blot都尽量要求使用单克隆抗体。以保证实验结果准确可靠
1.单克隆抗体的优点:
(1)杂交瘤可以在体外“永久”地存活并传代,只要不发生细胞株的基因突变,就可以不断的生产高特异性、高均一性的抗体.
(2)可以用相对不纯的抗原,获得大量高度特异的、均一的抗体.
(3)由于可能得到“无限量”的均一性抗体,所以适用于以标记抗体为特点的免疫学分析方法,如IRMA和ELISA等.
(4)由于单克隆抗体的高特异性和单一生物学功能,可用于体内的放射免疫显像和免疫导向治疗.
2.单克隆抗体的局限性:
(1)单克隆抗体固定的亲和性和局限的生物活性限制了它的应用范围.由于单克隆抗体不能进行沉淀和凝集反应,所以很多检测方法不能用单克隆抗体完成.
(2)单克隆抗体的反应强度不如多克隆抗体.
(3)制备技术复杂,而且费时费工,所以单克隆抗体的价格也较高.
制备抗体都需要进行纯化处理
清冷冻干燥后保存。


