FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5; Neutrophil-activating peptide ENA-78
- Product No.LAA860Hu81
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourceAntibody labeling
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelFITC
- Original Antibodyn/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IF.If the antibody is used in flow cytometry, please check FCM antibodies.
- Downloadn/a
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 95 For more details, please contact local distributors!US$ 221 For more details, please contact local distributors!US$ 316 For more details, please contact local distributors!US$ 790 For more details, please contact local distributors!US$ 3160 For more details, please contact local distributors!
SPECIFITY of the FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
The antibody isa rabbit polyclonal antibody raised against ENA78. It has been selected for its ability to recognize ENA78 in immunohistochemical staining and western blotting.
USAGE of the FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
Western blotting: 0.2-2µg/mL;1:250-2500 Immunohistochemistry: 5-20µg/mL;1:25-100 Immunocytochemistry: 5-20µg/mL;1:25-100 Optimal working dilutions must be determined by end user.
STORAGE of the of the FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Protein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitReal Time PCR Experimental Service
Related products
Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
APA860Hu01 | Active Epithelial Neutrophil Activating Peptide 78 (ENA78) | Cell culture; Activity Assays. |
RPA860Hu02 | Recombinant Epithelial Neutrophil Activating Peptide 78 (ENA78) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA860Hu03 | Recombinant Epithelial Neutrophil Activating Peptide 78 (ENA78) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA860Hu01 | Recombinant Epithelial Neutrophil Activating Peptide 78 (ENA78) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA860Hu02 | Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IP. |
PAA860Hu03 | Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IP. |
PAA860Hu01 | Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IP. |
LAA860Hu81 | FITC-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IF. |
LAA860Hu71 | Biotin-Linked Polyclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC. |
MAA860Hu22 | Monoclonal Antibody to Epithelial Neutrophil Activating Peptide 78 (ENA78) | WB; IHC; ICC; IP. |
SEA860Hu | ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA860Hu | CLIA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) | Chemiluminescent immunoassay for Antigen Detection. |
KSA860Hu01 | ELISA Kit DIY Materials for Epithelial Neutrophil Activating Peptide 78 (ENA78) | Main materials for "Do It (ELISA Kit) Yourself". |
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多抗,稀释度更大,特异性相对较差,容易出现多条带。
兔的单克隆抗体和鼠的单克隆抗体在使用上不会有什么区别。
用来很多抗体,许多时候觉得单抗多抗也未必是理论上那样的。单抗做不好的也有,多抗条带唯一且清晰的也有。
而且很多蛋白的抗体未必有那么多的选择。
其次,察看次目的蛋白的存在形式,有没有多聚体形式及变构形式;
最后,查看多家抗体公司的DATA,看看别人的WB做出来的条带的位置。
根据你说的,特异识别多个组织中的同样大小的条带,我觉得很可能就是你的目的蛋白。
单克隆抗体结构相似且均一,有高度特异性
又由于自然存在的抗原大都存在多个抗原表位,会刺激机体产生多种针对同一抗原的不同抗原表位相应的不同抗体.
抗原有两个基本特性,即抗原性和免疫原性。有抗原性的物质不一定有免疫原性,所以由此引出半抗原和完全全抗原,半抗原必须经过经过一定的改造(偶联蛋白载体BSA,OVA或者HSA等大分子物质)方能成为完全。一般而言完全抗原分子量越大(大于10KDa),结构越复杂引起免疫反应的能力也就越强。
抗体就是能与特异性抗原结合的免疫球蛋白,抗体一般分为多克隆抗体和单克隆抗体,多克隆抗体能与抗原的多个表位结合。本篇主要讲述兔来源的多克隆抗体的生产步骤
多抗一般制备流程:完全抗原的准备→兔子的免疫→ 效价检测和终放→抗体亲和纯化→抗体的浓缩和保存。