BackgroundProinflammatory cytokine tumor necrosis factor- (TNF- ) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF- , notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF- , notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively,(1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Synonyms: KUZ; MADM

Source

Immunogen: Synthetic peptide corresponding to amino acids 732 to 748 off the human ADAM10.Immungen sequence is identical in bovine and rat and differs from the mouse protein by one amino acid.

Product

Product Form: Unconjugated

Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

ApplicationsDetects ADAM10 by Western blot at 0.25 to 1 µg/ml.Detects an 85 kDa band can be detected in Jurkat cell lysates which may represent precursor protein.A faint 60 kDa band is also detected in some cell lines, including Jurkat, which may be processed mature protein.Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Jurkat whole cell lysate

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Rosendahl, M.S., et al., Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J. Biol. Chem. 1997, 272, 24588-245932. Pan, D. & Rubin, G.M., Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis. Cell 1997, 90, 271-2803. Qi, H., et al., Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science 1999, 283, 91-94 4. Howard, L., et al., Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types. Biochem. J. 1996, 317, 45-50

Protein Reference(s)

Database Name: UniProt

Accession number: O14672 (Human)O35598 (Mouse)Q10743 (Rat)

Species Accession: Human, Rat, Mouse

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf