Type
PolyclonalAntibody
Applications
Westernblotting
SourceofAntigen
E.coli
Hosts
Rabbit
Preparation
Theantibodywasraisedinrabbitsbyimmunizationwiththerecombinant HPG-1.
AminoAcidSequence
Theimmunizationantigen(16 kDa)isaproteincontaining136 AAofrecombinantHPG-1 N-TerminalHis-tag,9 extraAA(highlighted)
MKHHHHHHHMIKKNLKKLGIEETYLNIIKAIYDRPTASIQKTENLSTKTGTSQEYHLSPLWFYIVLEILASTIRQEKNLKDIHTEKEEVKLSLFADAMILYLKKPNDSTRKLLELIKKIHSSCRIKINIHFAFCSQ
SpeciesReactivity
Human.Notyettestedinotherspecies.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Add0.2 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.