Type
PolyclonalAntibody
Applications
Westernblotting,ELISA,Immunohistochemistry,Immunoprecipitation
AntibodiesApplications
SourceofAntigen
E.coli
Hosts
Rabbit
Preparation
TheantibodywasraisedinrabbitsbyimmunizationwiththerecombinantHumanREG-1alpha.
AminoAcidSequence
Theimmunizationantigen(17.8 kDa)isaproteincontaining156 AAofrecombinantHumanREG-1alpha.N-TerminalHis-tag12 AA(highlighted).
MKHHHHHHASHMQEAQTELPQARISCPEGTNAYRSYCYYFNEDRETWVDADLYCQNMNSGNLVSVLTQAEGAFVASLIKESGTDDFNVWIGLHDPKKNRRWHWSSGSLVSYKSWGIGAPSSVNPGYCVSLTSSTGFQKWKDVPCEDKFSFVCKFKN
TheaminoacidsequenceoftherecombinantHumanREG-1alphais100%homologoustotheaminoacidsequenceoftheHumanREG-1alphawithoutsignalsequence
SpeciesReactivity
Human.Notyettestedinotherspecies.
PurificationMethod
ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumanREG-1alpha.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.