Type
PolyclonalAntibody
Applications
Westernblotting,ELISA
SourceofAntigen
E.coli
Hosts
Rabbit
Preparation
TheantibodywasraisedinrabbitsbyimmunizationwiththerecombinantRatResistin.
AminoAcidSequence
Theimmunizationantigen(11.9 kDa)isaproteincontaining110 AAofrecombinantRatResistin.N-TerminalHis-tag16 AA(highlighted).
MRGSHHHHHHGMASHMPSMSLCPMDEAISKKINQDFSSLLPAAMKNTVLHCWSVSSRGRLASCPEGTTVTSCSCGSGCGSWDVREDTMCHCQCGSIDWTAARCCTLRVGS
SpeciesReactivity
Rat.Notyettestedinotherspecies.
PurificationMethod
ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantRatResistin.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Add0.2 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.