Type
PolyclonalAntibody
Applications
Westernblotting,ELISA,Immunohistochemistry
AntibodiesApplications
SourceofAntigen
E.coli
Hosts
Rabbit
Preparation
TheantibodywasraisedinrabbitsbyimmunizationwiththerecombinantHumanRELM-beta.
AminoAcidSequence
Theimmunizationantigen(11 kDa)isaproteincontaining102 AAofrecombinantHumanRELM-beta.C-TerminalHis-tag12 AA(highlighted).
MGSTQCSLDSVMDKKIKDVLNSLEYSPSPISKKLSCASVKSQGRPSSCPAGMAVTGCACGYGCGSWDVQLETTCHCQCSVVDWTTARCCHLTKLRSHHHHHH
TheaminoacidsequenceoftherecombinantHumanRELM-betais100%homologoustotheaminoacidsequenceoftheHumanRELM-betawithoutsignalsequence
SpeciesReactivity
Human.Notyettestedinotherspecies.
PurificationMethod
ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumanRELM-beta.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.