Ubiquitinisahighlyconservedregulatoryproteinexpressedinalleukaryotictissues.Originallythisproteinwascalled:UbiquitousImmunopoieticPolypeptide.Itsfunctionislabelingofproteinsfordegradationthroughubiquitinproteasomesystem(UPS).
Sourceofpositivecontrol,ubiquitinprotein:fulllengthrecombinantArABIdopsisthalianaubiquitin,ubq11,withN-terminal1XHis-3XHA-tag;locusaccession:At4g05050
storeproteinstandardat-20°C;Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfrommaterialadheringtothecaporsidesofthetubes.
AS08307|UBQ11|Ubiquitinantibodies
ProteinisprovidedinLaemliSDSloADIngbufferandshouldbeheatedupin98°Cfor5minutesbeforeloadingonthegel.
positivecontrol:a2.5-5μlloadperwellisoptimalformostchemiluminescentdetectionsystems.
10-12kDa
noconfirmedexceptionsfrompredictedreactivitycurrentlyknown
Concentration:0.8µg/µl.RecommendedloadpergeltoallowCoomasiestainingandwesternblotdetectionis2.5-5µl.
1xSDSLoadingBuffercontains:
tobeaddedwhenavailable
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