Donkeyanti-GoatIgG(H&L),DyLight®350ConjugatedisasecondaryantibodyconjugatedtoDyLight®350,whichbindstoGoatIgG(H&L)inimmunologicalassays.
DyLight®350hasAmax=353nm,Emax=432nm.AntibodiesarepurifiedusingsolidphaseGoatIgG(H&L)
DyLight®isaregisteredtrademarkofThermofisherInc.,anditssubsidaries.
PurifiedGoatIgG,wholemolecule
otherDyLight®anti-Goatsecondaryantibodies
Conjugateispresentin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree.0.05%(w/v)sodiumazideisaddedaspreservative.
Noreactivityisobservedto,non-immunoglobulingoatserumproteins.BSAandmilkhavetobereplacedbyotherblockingreagents,likedonekyserumorcommercialformulationswhicharefreefrombovineIgG.
1:20-1:2,000formostapplications
tobeaddedwhenavailable
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本人做多克隆抗体制备,所用蛋白为纯化的过敏原,与福氏佐剂等量混合,每次量为1mg,每次总的注射量为3-4ml,兔子皮下多点注射,但是其中两只兔子在首次免疫后死亡,另有一致在首次加强免疫后死亡,死亡原因全是消化不良,胃鼓的像气球一样,请问各位老师这是什么原因啊,应该如何改进啊?谢谢
如题,我做的wb标本来源是绵羊,一抗能选择goat来源的多克隆抗体吗?请各位大神多多指教!!
如题:破伤风抗毒素血清是属于多克隆抗体,还是单克隆抗体呢?请各位老师指教。
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