c-Jun (Phospho-Ser63) AntibodyPDF
Catalog Number:
E011001-2Amount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of serine 63 (L-T-SP-P-D).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
c-Jun (phospho-Ser63) antibody detects endogenous levels of c-Jun only when phosphorylated at serine 63.Reactivity:
Human, MouseApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100Swiss-Prot No.:
P05412References:
Wei W, et al. (2005). Cancer Cell. 8(1): 25-33.Sevilla A, et al. (2004). Oncogene.23 (55): 8950-8958.Li L, et al. (2004). J Biol Chem.279 (6): 4058-4065.Hurd C, et al. (2002). Oncogene.21 (14): 2154-2160.
P-Peptide - +Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (phospho- ser63) antibody (E011001).

UV - +Western blot analysis of extracts from NIH/3T3 cells using c-Jun (phospho-Ser63) antibody (E011001)
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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选用和特异性抗体同种亚型的,同样荧光素标记的抗体,采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合,那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚,是不需要同型对照的。另外,在多色同染的情况下,也不采用同型对照,而是采用FMO(fluorensence minus one)对照。
另外,对于同型对照的使用,也有很多反对意见。就算是同型抗体,也可能会有不同的亲和力,并不一定就能真实反应非特异性的结合。
二抗
抗体组合
免疫组化
免疫沉淀
ELISA
ELISPOT
流式
同型对照
酪胺信号放大
免疫对照/血清/阻断/封闭
Western Blot免疫印迹
免疫缓冲剂/固定/透化
细胞/组织裂解物
细胞核裂解物
细胞裂解物
组织抽提物
转染细胞裂解物
CBA蛋白定量
链霉亲和素
抗体纯化
其同型为;percp-cy5.5(25ug包装)浓度:0.2mg/ml,
那么如果CD45RA抗体如果加入20ul,那同型抗体应该加入多少ml:
我自己的初步计算是:1ul的3分之1.不知道我算对了吗?应该怎么加才好呢?
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像;
同形像统计图

