| Product Type | Blocking Peptide |
| Units | 50 µg |
| Host | Rabbit |
| Species reactivity | Human |
| Application | Western Blotting |
BackgroundBifunctional enzyme which acts as both a sphingolipid delta(4)-desaturase and a sphingolipid C4-hydroxylase.Up-regulated during keratinocyte differentiation. Not expressed at day 0 or day 3 after differentiation, detected on day 6 and increases by day 9.Belongs to the fatty acid desaturase family. DEGS subfamily.Highly expressed in skin, intestine and kidney.
Synonyms: DEGS2; Sphingolipid delta(4)-desaturase/C4-hydroxylase DES2
Source
Immunogen: Synthetic peptide derived from human DEGS2 protein.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline
Concentration: See vial for concentration
ApplicationsFor use with DEGS2 polyclonal antibodies (Cat. No. X2356P & X2357P).
Functional Analysis: Western Blotting
Positive Control: Highly expressed in skin, intestine and kidney.
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1. Mizutani, Y., et al. 'Identification of the human sphingolipid C4-hydroxylase, hDES2, and its up-regulation during keratinocyte differentiation.; FEBS Lett. 563:93-97(2004).2. Omae, F., et al. 'DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine.' Biochem J. 2004 May 1;379(Pt 3):687-95.3. Omae, F. et al. 'Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2.' FEBS Lett. 2004 Oct 8;576(1-2):63-7.4. Enomoto, A., et al. 'Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.' Biochem J. 2006 Jul 15;397(2):289-95.
Protein Reference(s)
Database Name: UniProt
Accession number: Q6QHC5 (Human)
Species Accession: Human
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选用和特异性抗体同种亚型的，同样荧光素标记的抗体，采用同样的染色步骤染同样浓度的细胞。然后上机检测。如果细胞有非特异性的结合，那同型对照的荧光强度可能会比未染色的阴性对照要高一些。
同型对照只用于那种阳性和阴性之间没有明确分解的情况。如果阳性细胞群非常清楚，是不需要同型对照的。另外，在多色同染的情况下，也不采用同型对照，而是采用FMO（fluorensence minus one）对照。
另外，对于同型对照的使用，也有很多反对意见。就算是同型抗体，也可能会有不同的亲和力，并不一定就能真实反应非特异性的结合。
二抗
抗体组合
免疫组化
免疫沉淀
ELISA
ELISPOT
流式
同型对照
酪胺信号放大
免疫对照/血清/阻断/封闭
Western Blot免疫印迹
免疫缓冲剂/固定/透化
细胞/组织裂解物
细胞核裂解物
细胞裂解物
组织抽提物
转染细胞裂解物
CBA蛋白定量
链霉亲和素
抗体纯化
其同型为;percp-cy5.5(25ug包装）浓度：0.2mg/ml,
那么如果CD45RA抗体如果加入20ul，那同型抗体应该加入多少ml:
我自己的初步计算是：1ul的3分之1.不知道我算对了吗？应该怎么加才好呢？
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像；
同形像统计图
