【求助】GAPDH可以作为胃癌临床样本组织western blot的内参抗体吗？

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2021-07-20

问题描述：

GAPDH可以作为胃癌临床样本组织westernblot的内参抗体吗？

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hehe201606

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应该可以

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wqdwqdqdwqd

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43retre应该可以但是有的文献已经报道了其在肿瘤组织中作为内参抗体，应该慎重考虑啊。。。

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China13113

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Glyceraldehyde3-phosphatedehydrogenase,commonlyknownasGAPDH,catalyzesthesixthstepofglycolysis,italsoparticipatesinnucleareventssuchastranscription,RNAbindingandtransport,DNAreplicationandrepair,aswellasapoptosis.Itsexpressionishighandconstantinmosttissuesandcelltypes,earningthegeneanditsproteinhousekeepingstatus.Becauseofthis,GAPDHiscommonlyusedasaproteinloADIngcontrolinWesternblotandinternalcontrolforRT-PCR.However,theamountofGAPDHexpressioncandifferbetweensometissuesanditssuitABIlityforyourexperimentshouldbetakenintoconsiderationbeforeselectingthistargetasacontrol.Pleasenotethatsomephysiologicalfactors,suchashypoxiaanddiabetes,increaseGAPDHexpressionincertaincellandtissuetypes.

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wqdwqdqdwqd

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展开引用China13113Glyceraldehyde3-phosphatedehydrogenase,commonlyknownasGAPDH,catalyzesthesixthstepofglycolysis,italsoparticipatesinnucleareventssuchastranscription,RNAbindingandtransport,DNAreplicationandrepair,aswellasapoptosis.Itsexpressionishighandconstantinmosttissuesandcelltypes,earningthegeneanditsproteinhousekeepingstatus.Becauseofthis,GAPDHiscommonlyusedasaproteinloADIngcontrolinWesternblotandinternalcontrolforRT-PCR.However,theamountofGAPDHexpressioncandifferbetweensometissuesanditssuitABIlityforyourexperimentshouldbetakenintoconsiderationbeforeselectingthistargetasacontrol.Pleasenotethatsomephysiologicalfactors,suchashypoxiaanddiabetes,increaseGAPDHexpressionincertaincellandtissuetypes.......谢谢大神的回复，但是在肿瘤组织中，是否有文献报道其作为内参的质疑呢？

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