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实时荧光定量pcr没出现扩增曲线为什么

来自 : mayitao


productinformation
Background		H3K9me3isahistonepost-translationalmodificationthatisenrichedintranscriptionallyinactiveregions.Itisalsoimportantincellulardifferentiationandmaturation.PhosphorylationofhistoneH3isalsoassociatedwithmitosisandmeiosis,andseemstoberelatedtodevelopmentalchanges.
Immunogen		KLH-conjugatedsyntheticpeptide
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinitypurifiedserum
Format
Quantity		50µg
Reconstitution
Storage		Storeat-20°C;makealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfrommaterialadheringtothecaporsidesofthetubes.
Testedapplications		Dotblot(Dot),chromatinimmunoprecipiation(ChIP),immunofluorescence(IF),immunohistochemistry(IHC),westernblot(WB)
Relatedproducts		AS163175\|anti-H3T6pK9me2\|HistoneH3(dimethylLys9,pThr6),rabbitantibodies AS163168\|anti-H3T3pK4me1\|HistoneH3(monomethylLys4,pThr3),rabbitantibodies AS163169\|anti-H3T3pK4me2\|HistoneH3(dimethylLys4,pThr3),rabbitantibodies AS163172\|anti-H3K4me3T6p\|H3(trimethylLys4,pThr6),rabbitantibodies AS163197\|anti-anti-H3core,monoclonalantibodies AS152855\|anti-H3\|histoneH3,chickenpolyclonalantibodies AS10710\|anti-H3\|histoneH3,rabbitpolyclonalantibodies AS10710PRE\|H3\|histoneH3,pre-immuneserumtoH3antibodyAS10710 collectionofantibodiestoepigenetics
Additionalinformation		Thisantibodypreparationisprovidedin20mMPotassiumPhosphatepH7.2,150mMNaCl,0,01%sodiumazideand30%glycerol.

applicationinformation
Recommendeddilution		2-5µg/millioncells(ChIP), 1:1000(Dot),1:100(IF),1:200(IHC),1:500(WB)
Expected\|apparentMW		15kDa
Confirmedreactivity		C.elegans,human
Predictedreactivity		chicken,D.melanogaster,mouse,plant,rat,Xenopussp.
Notreactivein		Noconfirmedexceptionsfrompredictedreactivityknownatthemoment
Additionalinformation		Tobeaddedwhenavailable
Selectedreferences		Tobeaddedwhenavailable,antibodyreleasedinFebruary2016.

applicationexample

DotBlotusinganti-H3T6pantibodies.Lane1:H3T6un.Lane2:H3T6p.Load:1,0.3,0.1,0.03picomolesofpeptide.Primaryantibodyusedata1:1000dilutionfor45minat4°C.Secondaryantibody:Dylight®488rabbitsecondaryantibodyat1:10000for45minatRT.
immunofluorescence using anti-H3T6pK9me3 | Histone H3 (trimethyl Lys9, p Thr6) antibodies

immunofluorescence using anti-H3T6pK9me3 | Histone H3 (trimethyl Lys9, p Thr6) antibodies

Immunofluorescenceusinganti-H3T6pantibodies.Tissue:HeLacells.Fixation:0.5%PFA.Primaryantibodyusedata1:100dilutionfor1hatRT.Secondaryantibody:FITCsecondaryantibodyat1:10,000for45minatRT.Localization:HistoneH3T6pisnuclearandchromosomal.Staining:H3T6pisexpressedingreenandthenucleiarecounterstainedwithDAPI(blue).

western blot using anti-H3T6pK9me3 | Histone H3 (trimethyl Lys9, p Thr6) polyclonal antibodies

western blot using anti-H3T6pK9me3 | Histone H3 (trimethyl Lys9, p Thr6) polyclonal antibodies

WesternBlotusinganti-H3T6pantibodies.30μgC.elegansembryolysate.Primaryantibodyincubatedata1:500dilutionovernightat4°C.Secondaryantibody:IRDye800™rabbitsecondaryantibodyat1:10000for45minatRT.

western blot using anti-H3T6pK9me3 | Histone H3 (trimethyl Lys9, p Thr6) polyclonal antibodies

WesternBlotofH3T6pantibodyon30μgHeLahistoneextracts.Primaryantibody:incubatedata1:500dilutionovernightat4°C.Secondaryantibody:IRDye800™rabbitsecondaryantibodyat1:10000for45minatRT.

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发布于： 2018-07-08 阅读（151）

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