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alpha-TubulinAntibodyProperties Description Anti-alpha-Tubulin(RABBIT)Antibody-600-401-880S Host Rabbit TargetSpecies Human KnownCrossReactivity human,mouse,rat.chicken,bovine Clonality Polyclonal Application ELISA:1:5,000 IFMicroscopy:1:500-1:2,000 WesternBlot:1:500-1:3,000 Immunohistochemistry:1:500-1:2,000 OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 1.1mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide Stabilizer None GeneName TUBA1B RelevantLinks NCBI-17986283 UniProtKB-P68363 |
alpha-TubulinAntibodyDescription
Background
TubulinLoadingControlAntibodyrecognizesmicrotubuleswhichareinvolvedinawidevarietyofcellularactivitiesrangingfrommitosisandtransporteventstocellmovementandthemaintenanceofcellshape.Tubulinitselfisaglobularproteinconsistingoftwopolypeptides(alphaandbetatubulin).Alphaandbetatubulindimersareassembledto13protofilamentsthatformamicrotubuleof22-nmdiameter.TyrosineligaseaddsaC-terminaltyrosinetomonomericalphatubulin.Assembledmicrotubulescanagainbedetyrosinatedbyacytoskeleton-associatedcarboxypeptidase.DetyrosinatedalphatubulinisreferredtoasGlu-tubulin.Anotherpost-translationalmodificationofdetyrosinatedalphatubulinisC-terminalpolyglutamylation,whichischaracteristicofmicrotubulesinneuronalcellsandthemitoticspindle.Thisantibodymakesanexcellentloadingcontrol.
Synonyms
rabbitanti-alpha-TubulinAntibody,rabbitanti-a-tubulinantibody,Tubulinalpha-1Bchain,Tubulinalpha-ubiquitouschain,Alpha-tubulinubiquitous,TubulinK-alpha-1,TUBA1B,tubulinloadingcontrol,Alpha-tubulin,Tubulinalpha-1A,TUBA1A,TUBA3,LIS3
Immunogen
Anti-TubulinLoadingControlAntibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaminoacids427-441ofHumanalphaTubulin.
ImmunogenType
Peptide
StorageCondition
Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.
ApplicationNote
Anti-TubulinAntibodyhasbeentestedforuseinELISAandwesternblot.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandat~50kDainsizecorrespondingtoalphatubulinbywesternblottinginmostcelllysatesorextracts.
Purity/Specifity
Anti-TubulinLoadingControlAntibodyisdirectedagainsthumanalphaTubulinprotein.TheLoadingControlAntibodywasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitypurification.ABLASTanalysiswasusedtosuggestthatthisantibodywouldreactwithalphaTubulinfromawiderangeoforganisms,includingavian,mammalianaquatic,parasiticandalgasourcesbasedon100%homologyfortheimmunogensequence.Crossreactivitywilloccurwithallisoformsofalphatubulin.Suchbroadreactivitymakesthisantibodyusefulasanexcellentloadingcontrol.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
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发布于 ： 2021-09-04
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