| productinformation
| | Background | | Actinisahighlyconservedproteinandanessentialcomponentofcellcytoskeletonandplaysanimportantroleincytoplasmicstreaming,cellshapedetermination,celldivision,organellemovementandextensiongrowth.Preferentiallyexpressedinyoungandexpandingtissues,floralorganprimordia,developingseedsandemerginginflorescence. | | Immunogen | | ca.100aminoacidsofrecombinantactin conservedmorethan80% inArabidopsisthaliana:actin-1P0CJ46 AT2G37620,actin-2Q96292AT3G18780,actin-3P0CJ47AT3G53750,actin-4P53494AT5G59370,actin-5Q8RYC2At2g42100,actin-7 P53492At5g09810,actin-8Q96293 AT1G49240,actin-11P53496,AT3G12110,actin-12P53497AT3G46520 | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Serum | | Format | | Lyophilized | | Quantity | | 50µl | | Reconstitution | | Forreconstitutionadd50µlofsterilewater. | | Storage | | Storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes. | | Testedapplications | | WesternBlot(WB),immunofluorescence(IF) | | Relatedproducts | | AS10702 |Anti-Actin-11,monclonalmouseantibody
AS10681|Anti-tubulinbetachain,rabbitantibody
AS10680|Anti-tubulinalphachain,rabbitantibody
AS163140|Actin-2,mousemonoclonalantibody
AS163141|Actin-8,mousemonoclonalantibody
AS163139|Actin-1,monoclonalantibody Plantproteinextractionbuffer Secondaryantibodies | | Additionalinformation | | Antibodyavailablein3variouspacksizes:50,100and150µl-please,inquire. |
applicationinformation
| | Recommendeddilution | | 1:3000-1:5000(WB),1:250(IF) | | Expected|apparentMW | | 41.6|45kDa | | Confirmedreactivity | | Agostisstolonifera cv.‘Penncross’, Arabidopsisthaliana,Cynaracardunculus,Glycinemax,Hordeumvulgare,Zeamays | | Predictedreactivity | | Agropyroncristatum,Betavulgaris,Betulaluminifera,Brassicanapus,Brassicarapasubsp.pekinensis,Capsellarubella,Castaneasativa,Chorisporabungeana,Cucumissativus,Cyanidioschyzonmerolaestrain10D,Glycinemax,Glycinesoja,Halogetonglomeratus,Medicagotruncatula,Malusdomestica,Nicotianatabacum,Oryzasativa,Pisumsativun,Solanumlysopersicum,Solanumtuberosum,Phaseolusvulgaris,Piceaabies,Piceasitchensis,Prunusavium,Ricinuscommunis,Rubusplicatus,Theobromacacao,Triticumaestivum, Viciafaba
| | Notreactivein | | Chlamydomonasreinhardtii(toohighbackgroundforthisspecies) | | Additionalinformation | | ThisproductcanbesoldcontainingProClinifrequested | | Selectedreferences | | Jespersenetal.(2017).MetabolicEffectsofAcibenzolar-S-MethylforImprovingHeatorDroughtStressinCreepingBentgrass.FrontPlantSci.2017Jul11;8:1224.doi:10.3389/fpls.2017.01224.eCollection2017.(westernblot,Agostisstoloniferacv.‘Penncross’)
Qiuetal.(2015).Soy14-3-3proteinSGF14c,anewregulatoroftolerancetosalt–alkalistress.PlantBiotechnologyReportspp1-9.
Shawetal.(2015).β-aminobutyricacidmediateddroughtstressalleviationinmaize(ZeamaysL.).EnvironSciPollutResInt.2015Sep29.
Buxaetal.(2015).Phytoplasmainfectionintomatoisassociatedwithre-organizationofplasmamembrane,ERstacks,andactinfilamentsinsieveelements.rontPlantSci.2015;6:650.Publishedonline2015Aug19.
Zhengetal.(2014).iTRAQ-basedquantitativeproteomicsanalysisrevealedalterationsofcarbohydratemetabolismpathwaysandmitochondrialproteinsinamalesterilecybridpummelo.JProteomeRes.2014May13. |
applicationexample | 
| 15µgoftotalproteinextractedwithPEB(AS08300)from leaftissueof(1)Arabidopsisthaliana,(2)Hordeumvulgare,(3)Zeamayswereseparatedon4-12%NuPage (Invitrogen)LDS-PAGEandblotted1htoPVDF.Filterswereblocked1hwith2%low-fatmilkpowderinTBS-T(0.1%TWEEN20)andprobedwithanti-actin(AS132640,1:2500,1h)andsecondaryanti-rabbit(1:10000,1h)antibody(HRPconjugated,recommendedsecondaryantibodyAS09602)inTBS-Tcontaining2%lowfatmilkpowder.AntibodyincubationswerefollowedbywashingsinTBS-T(15,+5,+5,+5min).AllstepswereperformedatRTwithagitation.SignalwasdetectedwithECLAdvance(GEHealthcare)usingaFujiLAS-3000CCD(300s,standardsensitivity).Exposuretimewas2min.
|
 Actincytoskeletonin5daysoldArabidopsisthalianaseedlings.Actinsignalshowningreen,PIN1inredandDAPIinblue.Thematerialhasbeenfixedin2%formaldehydefor45minutes.Tissuecleaninghasbeenperformedbeforeimmunolocalization.Rabbitanti-actinprimaryantibodywasdilutedin1:250andanti-rabbitAlexa488andAlexa555werebothdilutedin1:500(Invitrogen).Scalebar-20µm. Courtesy:Dr.TarasPasternak
|
