
FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1)
ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta
- Product No.LAA032Ra81
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourceAntibody labeling
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelFITC
- Original Antibodyn/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IF.If the antibody is used in flow cytometry, please check FCM antibodies.
- Downloadn/a
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 143 For more details, please contact local distributors!US$ 335 For more details, please contact local distributors!US$ 478 For more details, please contact local distributors!US$ 1195 For more details, please contact local distributors!US$ 4780 For more details, please contact local distributors!
SPECIFITY of the FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1)
The antibody isa rabbit polyclonal antibody raised against FGF1. It has been selected for its ability to recognize FGF1 in immunohistochemical staining and western blotting.
USAGE of the FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1)
Western blotting: 0.2-2µg/mL;1:250-2500 Immunohistochemistry: 5-20µg/mL;1:25-100 Immunocytochemistry: 5-20µg/mL;1:25-100 Optimal working dilutions must be determined by end user.
STORAGE of the of the FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Protein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitReal Time PCR Experimental Service
Related products
Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
RPA032Ra01 | Recombinant Fibroblast Growth Factor 1, Acidic (FGF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA032Ra01 | Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) | WB; IHC; ICC; IP. |
LAA032Ra81 | FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) | WB; IHC; ICC; IF. |
LAA032Ra71 | Biotin-Linked Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) | WB; IHC; ICC. |
MAA032Ra21 | Monoclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) | WB; IHC; ICC; IP. |
SEA032Ra | ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA032Ra | CLIA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) | Chemiluminescent immunoassay for Antigen Detection. |
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你用BSA封闭没什么意思,可以换一种,比如明胶,酪蛋白等。
将HRP标记BSA稀释成一定的梯度。
(2)6 X His tag 序列:CAT CAT CAC CAT CAC CAT
(3)GST标签序列:
1ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGC
301 AACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCAT
351 TTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATT
401 GGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAAT
451 TAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATG
501 TTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGC
551 GGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACT
601 TTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAA
651 ATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGT
701 AACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACA
751 TGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAA
801 CGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTA
851 TATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACC
901 ATCCTCCAAAATCGGATCTGGTTCCGCGTGGATCCCCGGAATTCCCGGGT
951 CGACTCGAGCGGCCGCATCGTGACTGA
百度上的,,,,,搬运
Flag标签系统是已被公认的用于表达、纯化以及检测融合蛋白的表达系统,广泛应用于Westernblotting、免疫细胞组化、免疫共沉淀、流式细胞术、蛋白纯化以及蛋白相互作用、蛋白分离等多个研究领域。Flag标签是一个与重组蛋白相融合的由8个亲水氨基酸(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)组成的多肽片断。FLAG标签只有8个氨基酸组成,因此它不会占据其他表位与结合域,避免导致融合蛋白的功能、分泌性以及转运发生改变。由于它的亲水性特点,FLAG标签倾向于定位在融合蛋白表面,因此更易与抗体结合以及被肠激酶酶解。
由于Flag标签可位于蛋白质的C端或N端,这一系统已用于各种细胞类型,包括细菌、酵母和哺乳细胞。由于Flag标签系统的纯化条件是非变性的,因此可以纯化所有有活性的融合蛋白。EarthOx的Anti-FlagTagMonoclonalAntibody可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位,以及纯化、定性或定量检测Flag融合表达蛋白等。比如在免疫荧光IF的实验里,同时配合EarthOx的Dylight549荧光二抗,使用该抗体可以准确的定位出Flag融合表达蛋白在细胞内的位置(如图红色显示部分)。另外,做融合表达分析前,通过免疫共沉淀技术获得Flag融合表达蛋白也是非常重要的一步,因此选择一个合适的、可用于免疫共沉淀(Immunoprecipitation,IP)Flag标签抗体也是很必要的。EarthOx的Anti-FlagTagMonoclonalAntibody不仅能应用于细胞内定位的IF实验,同样还能应用于IP实验。1:200甚至更高的IP实验稀释比率,也说明了该抗体本身的具有很高的效价,同时,因为是单克隆抗体,因此特异性也很高。高效价所对应的高稀释比率也意味着超高性价比。
抗体指机体的免疫系统在抗原刺激下,由B淋巴细胞分化成的浆细胞所产生的、可与相应抗原发生特异性结合反应的免疫球蛋白.
载体,你说的是那种载体?生命科学载体有2种,一种是分布于膜上的载体蛋白,和物质运输有关.另一种是基因工程里面的携带外源DNA的一段DNA片段,例如质粒、噬菌体和动植物病毒.
所以,抗体全部是蛋白质,载体部分是蛋白质.

