The ImmunoStar Gastrin Releasing Peptide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin.
Photo Description: IHC of at stomach staining for gastrin releasing peptide. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and counterstained with hematoxylin
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Chick, Hamster, Human, Mouse, Newt, Pigeon, Quail, Rabbit, Ram (Sheep), Rat, Zacco Platypus (Minnow)
Availability: In Stock
Preabsorption Control: Available at irbiologicals, Catalog # IRCP069. Sequence: Glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH
Alternate Names: BN; GRP-10; proGRP; preproGRP; gastrin-releasing peptide, anti-GRP, Bombesin, anti-Bombesin
Gene Symbo: GRPRRID: AB_572221Database Links: Entrez Gene: 425213 Chick Entrez Gene: 100769410 Hamster Entrez Gene: 2922 Human Entrez Gene: 225642 Mouse Entrez Gene: 102093899 Pigeon Entrez Gene: 107325907 Quail Entrez Gene: 103349406 Rabbit Entrez Gene: 443332 Ram (Sheep) Entrez Gene: 171101 Rat
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单抗标签选择的一抗范围比较小一些，多抗标签选择的一抗范围可能相对大一些。而商品化二抗基本没什么差别。不管是选择什么样的抗体，在Western blot检测方面都是一样的
所以做Western blot的单抗和多抗标签并没有什么不同
例如 GST (1-109):
•epitope corresponding to amino acids 1-109 mapping at the N-terminus of GST of Schistosoma japonicum origin
•recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
还有Schistosoma japonicum origin 跟我载体pGEX4T-1表达的融合蛋白可用Western Blot吗?它们有什么关系？
十分感谢
抗体指机体的免疫系统在抗原刺激下,由B淋巴细胞分化成的浆细胞所产生的、可与相应抗原发生特异性结合反应的免疫球蛋白.
载体,你说的是那种载体?生命科学载体有2种,一种是分布于膜上的载体蛋白,和物质运输有关.另一种是基因工程里面的携带外源DNA的一段DNA片段,例如质粒、噬菌体和动植物病毒.
所以,抗体全部是蛋白质,载体部分是蛋白质.
选择Flag标签抗体，主要根据实验的需要。一个抗体检测后应用类型越多，使用中选择余地就越大。要综合考虑抗体来源，特异性，稳定性，效价等等因素
想做个蛋白的定位，可是没有好抗体，就想做个转基因小鼠，在基因后面加一个标签。但是之前没有相关经验，不知道哪一种tag更好呢，更适合在小鼠里表达呢？因为我蛋白比较小，加GFP那么大的话可能会影响表达。比较小的tag例如HA，Flag还有His等，不知道哪个更适合一些？求大神们给些经验。
