| 货号： | AT0079 |
| 供应商： | 全国独家总代上海维景生物 |
| 英文名： | antiHAaffinitygel |
| 规格： | 1ml,10ml |
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AgarosebeadsConjugatedanti-HAMousemonoclonalantibody
Catalognumber：AT0079 Size：1ml
Specificity： Anti-HAAgarosebeads(Affinitygel)isamousemonoclonalantibodythatiscovalentlyattachedtoagarosebeadsbyhydrazidelinkage.TheantibodybindsHAattheN-terminal,C-terminalandinternallocationsoffusionproteins.
Testedapplications: Immunoprecipitation(IP),
40mlofgelsuspension(~20μlofpackedbeads)for~1000μgofcrudetotalproteinsolution.
Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
Reactivity: TheantibodydetectsandcapturesHA-TaggedfusionproteinsoverexpressedinmammaliancellsorE.coli.
Immunogen: Thismonoclonalantibodyisproducedbyimmunizingmouseswithasyntheticpeptide(KLH-coupled)containingYPYDVPDYA.
Form: Theproductissuppliedasa50%suspensionin50%glycerolwith10mMsodiumphosphateand150mMsodiumchloride,pH7.4,containing0.02%(w/v)sodiumazide
Binding capacity: 30-50nmolesofHA-taggedfusionproteinper1mlofsettledgel.
Isotype: MouseIgG1
RecommendedIPLysisbufferformammaliancells: 50mMTrisHCl,pH7.4,with150mMNaCl,1mMEDTA,and1%TRITONX-100.
Storageinstruction:
Thegelissuppliedin50%glycerol,sothegelcanbestoredat–20°Cformaximumstability.Theunopenedproductisstableforoneyearwhenstoredasindicated.
Forusedandrecycledgel,thebeadsshouldbecleanedandstoredin50%glycerolwithTBSorPBSbuffercontaining0.02%sodiumazidetoprotecttheproduct.Storedat–20°Cformaximumstabilityor4°Cforshortterm(1-2weeks).Donotfreezeintheabsenceofglycerol.
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