H3K4me3MouseMonoclonalAntibody
Clonality:MouseIG1 | Source:Mouse | |
Applications:WB:1:500-1:2000 | Cross-Reactivity:HMRat | |
Concentration:100ug/100ul | Detectedband(bywestern)MW:15KD | |
Alt.Names
H3histoneantibody,HIST1H3Aantibody,Histonecluster1,H3aantibody
Specificity:
TheHistoneH3(trimethylK4)mousemonoclonalantibodycanrecognizeendogenousHistoneH3(trimethylK4)proteins.
Immunogen:
MousemonoclonalantibodyispreparedbyimmunizingsyntheticpeptidecoupledtoKLH.
StorageConditionandBuffer:
Storeat-20°C.Stableforoneyearfromthedateofshipment.1mg/mlinPBS,pH7.4,containing0.02%sodiumazideand50%glycerol
Westernblotanalysisof1)Hela,2)RatTestistissue,3)Raw264.7withHistoneH3(trimethylK4)MousemAbdilutedat1:1000.
H3K4methylationisoneofthegeneactivationMarkersandoneofthehottestepigenetictargetsinrecentstudies.H3K4me1,H3K4me2andH3K4me3haveacommonalityforgeneactivation,althoughtheirsubsetsplaydistinctrolesinmodulatingchromatinfunction(4).Amongwhich,H3K4me3occupiesasmanyas75%ofallhumangenepromotersinseveralcelltypes(e.g.,EScells),playingimportantroleinmammaliangeneexpression(1).H3K4me2decoratesgenomicregionsindependentlyofH3K4me3,althoughmostofitoverlapswithH3K4me3nearthetranscriptionstartsites(2).MonomethylatedH3K4(H3K4me1)alsoco-occupiesregionsnearthestartsiteswithH3K4me3.Apartfromthetranscriptionstartsite,H3K4me1,togetherwithH2K27acetylation,specifiesenhancerregions(3).
Inrecentstudies,H3K4hasbeendemonstratedtoberelatedstemcellself-renewalanddifferentiation(4).
H3K4methylationstatusescanbedetectedbyourH3K4methylationantibodyseries.
H3K4me3_monocolonal_Antibody.pdf
SDS_H3K4me3_Mouse_Monoclonal_Antibody.pdf
References:
- PanG,TianS,NieJ,ThomsonJA:Whole-genomeanalysisofhistoneH3lysine4andLysine27.CellStemCell2007,1:299-312.
- 2.BernsteinBE,KamalM,Lindblad-TohK,GingerasTR,etal:Genomicmapsandcomparativeanalysisofhistonemodificationsinhumanandmouse.Cell2005,120:169-181.
- 3.ViselA,BlowMJ,Liz,ZhangT,WrightC,ChenF,etal:CHIP-seqaccuratelypredictstissue-specificactivityofenhancers.Nature2009,457:854-858.
- 4.BingnanGuandMinGyuLee:HsitoneH3lysine4methyltransferasesanddemethylasesinself-renewalanddifferentiationofstemcells.Cell&Biosciences2013,3:39.
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第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
