Lyophilized Powder This product is freeze dried. All water molecules have been removed.
Antigen Incl. This antibody is shipped with its antigen FREE of charge!
- Peptide CRDPDYGTLIQE, corresponding to amino acid residues 27-38 of rat RAMP1 (Accession Q9JJ74). Extracellular, N-terminus.
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Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat pancreas (lanes 3 and 6):1-3. Anti-RAMP1 (extracellular) Antibody (#ARR-021).
4-6. Anti-RAMP1 (extracellular) Antibody, preincubated with the negative control antigen.
The antibody was used at a concentration of 1:400 for brain lysates and 1:200 for pancreas lysates.
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Expression of RAMP1 in rat PC12 cellsCell surface detection of RAMP1 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-RAMP1 (extracellular) Antibody (#ARR-021), (1:50), (red). B. Live view of cells.
- 1. Kadmiel, M. et al. (2012) Adv. Exp. Med. Biol. 2012, 744, 49.
- 2. Jacob, A. et al. (2012) Adv. Exp. Med. Biol. 744, 87.
- 3. Conner, A.C. et al. (2004) Biochem. Soc. Trans. 32, 843.
- 4. Li, M. et al. (2014) PLoS One 9, e102356.
- 5. Shi, B. et al. (2014) Exp. Biol. Med. 239, 356.
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Receptor activity modifying protein-1 (RAMP1) is part of a three-member group (that further includes RAMP2 and RAMP3 – each encoded by a different gene) of transmembrane proteins that interact with G-protein coupled receptors (GPCRs) such as the calcitonin receptor-like receptors (CLRs)1. This interaction leads to the formation of the receptor for calcitonin gene related peptide (CGRP) and is necessary for the binding of the peptide to the receptor. The binding of RAMP2 and RAMP3 to CLR, on the other hand, creates the receptor for adrenomedullin, a peptide functionally and structurally related to calcitonin1,2.
RAMP1 (as well as the two other RAMPs) has a short intracellular domain and a single transmembrane domain. Its extracellular region contains four conserved cysteine residues that putatively form two disulfide bonds. This domain confers specificity to the receptor complex, whereas the transmembrane chain allows the association of the RAMPs with the GPCRs. In contrast to the other RAMPs, RAMP1 lacks glycosylation sites and therefore is unable to migrate to the cell surface independently. To reach the cell surface, it aggregates inside the cytoplasm and forms heterodimers with calcitonin3.
RAMP1 is highly prominent in the neuronal tissues (especially the trigeminal ganglion, striatum, cortex, olfactory bulb, dorsal root ganglion, and the spinal cord) but abundant as well in the electrical conducting system of the heart, blood vessels, and related sites1,2,4.
While not dramatically affected, RAMP1-KO mice are reported to have higher blood pressure and to suffer from repressed wound healing process, while overexpression of RAMP1 in animals leads to extravasation and vasodilation2. Interestingly, asthmatic symptoms in mice model of asthma were mitigated in mice that were RAMP1-deficient4. Furthermore mesenchymal stem cells overexpressing RAMP1 were shown to improve cardiac function and to reduce occurrences of over-thickened blood vessels5.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat RAMP1. Anti–RAMP1 (extracellular) Antibody (#ARR-021) can be used in western blot and live cell imaging applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize RAMP1 from rat and mouse samples. The antibody does not recognize RAMP1 from human samples.
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因此所有羊抗兔可以结合兔子的同种异型抗体，也就是说只要是兔子产生的抗体，羊抗兔（也叫抗抗体或者2抗）都可与之结合。
在试验中，二抗经常被做了手脚，打上了标记，比如辣根过氧化物酶，经常被用在ELISA，WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属：兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记：HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应：鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用：用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验
