Capturem Protein A columns and plates provide rapid, high-quality, small-scale purification and screening of monoclonal and polyclonal antibodies. This purification method combines the specific antibody-binding properties of Protein A with novel Capturem technology to provide high-capacity, membrane-based affinity purification. Where resin columns fall short, Capturem Protein A yields highly pure monoclonal and polyclonal antibodies in just 5–15 minutes, from loading the sample through elution.
Capturem Protein A columns and plates provide rapid, high-quality, small-scale purification and screening of monoclonal and polyclonal antibodies. This purification method combines the specific antibody-binding properties of Protein A with novel Capturem technology to provide high-capacity, membrane-based affinity purification. Where resin columns fall short, Capturem Protein A yields highly pure monoclonal and polyclonal antibodies in just 5–15 minutes, from loading the sample through elution. Short residence times greatly reduce the possibility of antibody aggregation or loss of activity, giving downstream applications a higher probability of success.
Protein A is a cell wall protein from the bacterium Staphylococcus aureus with a high affinity for the Fc region of monoclonal and polyclonal IgG-type antibodies from several different species, including mouse, rat, and human. Capturem kits use a novel high-capacity membrane-based affinity technology that has previously been used to purify highly concentrated his-tagged proteins in just minutes at room temperature, via miniprep columns, maxiprep columns, 24-well plates, and 96-well plates.
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因此所有羊抗兔可以结合兔子的同种异型抗体，也就是说只要是兔子产生的抗体，羊抗兔（也叫抗抗体或者2抗）都可与之结合。
在试验中，二抗经常被做了手脚，打上了标记，比如辣根过氧化物酶，经常被用在ELISA，WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属：兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记：HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应：鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用：用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验
