Theanti-mouseCD3F(ab)2fragmentistheantigenbindingF(ab)2fragmentsofthe145-2C11monoclonalantibodyonly.ThemajorityoftheFcfragmenthasbeenremovedviapepsindigestion.The145-2C11antibodyF(ab)2fragmentreactswithmouseCD3ε,a20kDatransmembranecell-surfaceproteinthatbelongstotheimmunoglobulinsuperfamily.CD3εisoneoffivepolypeptidechainsthatcombinetoformtheTCRcomplex.CD3εisexpressedonTlymphocytes,NK-Tcells,andtovaryingdegreesondevelopingthymocytes.CD3playsrolesinTCRsignaling,Tlymphocyteactivation,andantigenrecognition.Thecomplete145-2C11antibodyiscommonlyusedtostimulateTcellsinvitrohowever,whenusedinvivoactivationofrestingTcellsresultsincytokinereleaseandtoxicitycausedbyAb-mediatedcross-linkingofTcellsandFcγreceptor-bearingcells.Toavoidthesecomplicationsnon-FcreceptorbindingF(ab’)2fragmentsofthe145-2C11antibodyarecommonlyused.Thisnon-FcR-bindinganti-CD3inducesapoptosisofAg-activatedTcellsinvivo byallowingdurableexpressionoftheTCRandsustainedsignaling.Foxp3+TregshavebeenshowntoberesistanttoCD3antibody-mediateddepletion.
| Isotype | ArmenianHamsterIgG1 |
| RecomendedIsotypeControl(s) | InVivoMAbhamsterIgGf(ab)2fragments |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | MouseBM10-37cytotoxicTcells |
| ReportedApplications | invivoTcelldepletion |
| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | PepsinDigest |
| Purification | ProteinA |
| Storage | Undilutedat4°Cinthedark |
