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Clone/ABfeature:

(#16R6)

Antigen:

MouseR‑Spondin4

Stability:

Lyophilizedsamplesarestablefor2yearsfromdateofreceiptwhenstoredat-70°C.Reconstitutedantibodycanbealiquotedandstoredfrozenat< -20 °C for at least for six months without detectable loss of activity.

Reconstitution:

Reconstitutetheantibodywith500µlsterilePBSandthefinalconcentrationis200µg/ml.

Description:

TheR-Spondin(Rspo)proteinsbelongtotheRspofamilyofWntmodulators.Currently,thefamilyconsistsoffourstructurallyrelatedsecretedligands(Rspo1-4),allcontainingfurin-likeandthrombospondinstructuraldomains.TheRspoproteinscaninteractwiththeFrizzled/LRP6receptorcomplexinamannerthatcausesthestabilizationandresultingaccumulationoftheintracellularsignalingprotein,β-catenin.Thisactivityeffectivelyactivatesandincreasesthesubsequentnuclearsignalingofβ-catenin.R-SpondincanalsobindtothepreviouslydiscoveredG-proteincoupledreceptors,LGR-4andLGR-5.Rspo/β-cateninsignalingcanactasaninducerofthetransformedphenotype,andcanalsoregulatetheproliferationanddifferentiationofcertainstemcellpopulations. RecombinanthumanR-Spondin-3isa26.9kDaproteinconsistingof240aminoacidresidues. Duetoglycosylation,R-Spondin-3migratesatanapparentmolecularweightofapproximately37.0kDabySDSPAGEanalysisunderreducingconditions.
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