TheY13-238monoclonalantibodyreactswithhuman,mouse,andratv-H-ras(withinaminoacids120-138)anddoesnotreactwithv-K-ras.v-H-rasbindsGTP/GDPandhasintrinsicGTPaseactivity.RasproteinsalternatebetweenaninactiveformboundtoGDPandanactiveformboundtoGTP,activatedbyaguaninenucleotide-exchangefactor(GEF)andinactivatedbyaGTPase-activatingprotein(GAP).Undernormalconditions,Rasfamilymembersinfluencecellgrowthanddifferentiationevents.MutationsintheRasfamilyofproto-oncogenesareverycommon,beingfoundin20%to30%ofallhumantumors.Raspointmutationsarethesinglemostcommonabnormalityofhumanproto-oncogenes.TheY13-238antibodydoesnotneutralizerasGTPasebindingandhydrolysisinvivoorinvitro.
Isotype | RatIgG2a |
RecomendedIsotypeControl(s) | InVivoMAbratIgG2aisotypecontrol,anti-trinitrophenol |
RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
Immunogen | HarveymurinesarcomavirusinfectedNRKcells |
ReportedApplications | Immunoprecipitation |
Endotoxin |
|
Purity |
|
Formulation |
|
Sterility | 0.2μMfiltered |
Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
Purification | ProteinG |
Storage | Undilutedat4°Cinthedark |