Domains Category : A(1186) A 单词类别
来自 : mayitao
| productinformation
| | Background | | Donkeyanti-SheepIgG(H&L)-DyLight®550Conjugated,min.crossreactivitytohumanorrabbitIgG isasecondaryantibodyconjugatedto DyLight®550,whichbindstoall Sheep IgG (H&L) inimmunologicalassays. DyLight®550hasAmax=562nm,Emax=576nm.Antibodiesareareaffinitypurifiedusingsolidphase SheepIgG(H&L). DyLight®isaregisteredtrademarkofThermofisherInc.,anditssubsidaries. | | Immunogen | | PurifiedSheepIgG,wholemolecule | | Host | | Donkey | | Clonality | | Polyclonal | | Clone | | | | Purity | | AffinitypurifiedDonkeyIgG | | Format | | Lyophilized | | Quantity | | 1mg | | Reconstitution | | Forreconstitutionadd1.1mlofsterilewater.Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily. | | Storage | | Storelyophilizedmaterialat2-8°C.Productisstablefor4weeksat2-8°Cafterrehydration.
Forlongtimestorageafterreconstitution,dilutetheantibodysolutionwithglyceroltoafinalconcentrationof50%glycerolandstoreasliquidat-20°C,topreventlossofenzymaticactivity.
Forexample,ifyouhavereconstituted1mgofantibodyin1.1mlofsterilewateradd1.1mlofglycerol.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming. | | Testedapplications | | | | Relatedproducts | | otherdylightanti-sheep secondaryantibodies | | Additionalinformation | | Conjugateispresentin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree.0.05%(w/v)sodiumazideisaddedaspreservative. |
applicationinformation
| | Recommendeddilution | | 1:20 -1:2,000formostapplications | | Expected|apparentMW | | | | Confirmedreactivity | | | | Predictedreactivity | | | | Notreactivein | | | | Additionalinformation | | Basedonimmunoelectrophoresis,thisantibodyreactswith:heavy(γ)chainsonsheepIgGlightchainsonallsheepimmunoglobulins. Noreactivityisobservedto:non-immunoglobulinsheepserumproteinsIgGfromhumanorrabbit. BSAandmilkhavetobereplacedbyotherblockingreagents,likedonekyserumorcommercialformulationswhicharefreefrombovineIgG. | | Selectedreferences | | tobeaddedwhenavailable |
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