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来自 : mayitao
| productinformation
| Background | | Donkeyanti-sheepIgG(H&L),FITC(fluorescein-5-isothiocyanate)conjugated,isasecondaryantibodywhichbindstoallsheepIgGsinimmunologicalassays. FITChasAmax=494nm,Emax=518nm.Fluortoproteinratiois3-7molesFITCpermoleantibody.Antibodyisadsorbedagainst human,mouse,rabbitIgGandaffinitypurifiedusingsolidphasesheepIgG. | Host | | Donkey | Clonality | | Polyclonal | Clone | | | Purity | | AffinitypurifiedIgG | Format | | Liquid | Quantity | | 1mg | Reconstitution | | | Storage | | Storeat2-8°C.Shelflifeofthisproductisoneyearfromdateofreceipt. | Testedapplications | | | Relatedproducts | | otherfluorescentdyeconjugatedsecondaryantibodies | Additionalinformation | | ThisantibodyreactswiththeheavychainsonsheepIgGandwiththelightchainsonallsheepimmunoglobulinsbasedonimmunoelectrophoresis. Minimumcross-reactivityisobservedtonon-immunoglobulinsheepserumproteinsandhuman,mouse,rabbitIgGbasedonimmunoelectrophoresis. Antibodyissuppliedin10mMsodiumphosphate,150mMsodiumchloride,pH7,1%(w/v)BSA,Protease/IgGfreeand0.05%(w/v)sodiumazideaspreservative.
BSAandmilkhavetobereplacedbyotherblockingreagents,likedonekyserumorcommercialformulationswhicharefreefrombovineIgG. |
applicationinformation
| Recommendeddilution | | 1:20-1:2000forimmunohistochemistry(IHC),1:50-1:5000forimmunocytochemistry(ICC)andforflowcytometry. | Expected|apparentMW | | | Confirmedreactivity | | | Predictedreactivity | | | Notreactivein | | | Additionalinformation | | | Selectedreferences | | |
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