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恒温振荡培养箱的使用及维护保养方法看这些就够了
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productinformation
Background

Donkey anti-sheepIgGisasecondaryantibodyconjugatedtoHRP(horseradishperoxidase)whichbindstoallsheepimmunoglobulinsinimmunologicalassays.

Immunogen

PurifiedsheepIgG,wholemolecule

HostDonkey
ClonalityPolyclonal
Clone
PurityPurifieddonkeyIgG
FormatLyophilized
Quantity1mg
Reconstitution
StorageStorelyophilizedmaterialat2-8°C.Forstorageat-20°Cafterreconstitutiondiluteantibodysolutionwithanequalvolumeofglyceroltoobtainfinalglycerolconcentrationof50%topreventlossofenzymaticactivity.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.
TestedapplicationsELISA(ELISA),westernblot(WB),immunohistochemistry(IHC)
Relatedproducts

AS09611|Donkeyanti-sheepIgG(H&L)biotinconjugated

Additionalinformation

Antibodyconcentrationis1mg/mlandithasbeenpurifiedbyantigen-specificchromatography

HRP-conjugateissuppliedin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,10%(w/v)BSA,Protease/IgGfree

0.1%(v/v)ofKathonCGisusedaspreservative.

BSAandmilkhavetobereplacedbyotherblockingreagents,like
donekyserumorcommercialformulationswhicharefreefrombovineIgG.

applicationinformation
Recommendeddilution

1:200-1:5000(ELISA),1:200-1:5000withregularECL(WB),1:500-1:5000(IHC)

Expected|apparentMW
ConfirmedreactivitysheepIgGheavyandlightchains(H&L)
Predictedreactivity

sheepIgGheavyandlightchains(H&L)

Notreactivein

noconfirmedexceptionsfrompredictedreactivityknowninthemoment

Additionalinformation

Noreactivityisobservedtonon-immunoglobulinsheepserumproteinsbasedinimmunoelectrophoresis

Selectedreferences

tobeaddedwhenavailable



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