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Spore-EX Spray is a reliable, all-around solution for the highly effective surface disinfection of critical workspaces in less than 1 minute. It has a scientifically tested bactericidal, virucidal, fungicidal, tuberculocidal and sporicidal efficacy meeting all EU requirements including EN1276; EN1650; EN14348; EN13704; EN14476; EN13727; EN13697; and EN13624. Spore-EX eliminates spores, viruses, bacteria and fungi (including Clostridium difficile, Hepatitis B/C, H1N1, MRSA, TB and NDM-1) reliably within 1 minute.Spore-EX is very safe and simple in its use. It is a unique formulation containing only a very low concentration of isopropanol and is free of aldehydes, chlorine, phenols, peroxides as well as nonylphenol ethoxylates (NPEs). Spore-EX is dermatologically tested and not known to be mutagenic or cancerogenic. Moreover, it is non-abrasive, non-volatile and non-corrosive making it safe to use on all washable, hard surfaces. Thus, Spore-EX Spray is excellent for cleaning and disinfection of hard laboratory surfaces and apparatuses, including laminar flow hoods, incubators, work benches, cell storage boxes, cryo-chambers, or instruments – making it ideal for laboratory everyday use in order to maintain a safe, clean, contamination-free work area.Aquaguard-1, a non-volatile water stabilizer/cleaner, is extremely effective at maintaining the water quality in CO2 incubators (tray). In addition, it does not cause damage to the stainless steel of the incubator or any plastic interior, and it does not cause any skin irritation when used as recommended. It comes as concentrated ready-to-use solution.Aquaguard-2 is intended for maintenance of water quality in ordinary water baths. The active ingredient in Aquaguard-2 is highly effective to prevent growth of unwanted microorganisms such as bacteria and fungi. It is non-volatile, non-irritant and non-corrosive. Aquaguard-2 is stable at high temperatures (100°C) and does not produce foam in circulating water baths. It comes as concentrated ready-to-use solution.Mycoplasma Disinfection Kit II not available in the US and Canada!
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求速度考试，简答
链接很容易，就用EDC或者CMC等碳二亚胺，是最容易在羧基和氨基之间催化形成酰胺键，反应条件就是0到4度，过夜，用磁力搅拌子温和搅拌，pH<7反应快，不过pH=7或稍微pH>7也关系不大。EDC或者CMC等碳二亚胺催化活性极高，而且一般的蛋白质的分子表面总有Lys,Arg,Asn,Gln，所以游离在分子表面的羧基和氨基总是不少的，参与反应的两种蛋白质用大致10：1的比例混合（哪种蛋白质便宜，哪种蛋白质的物质的量就大些，但是这不是全部原因），反应样品浓度就按照平时使用时的浓度或略微更低浓度（别用储存液的浓度）即可。很容易反应，而且EDC或者CMC等碳二亚胺是零臂连接试剂，用于空间位阻，形成多分子交联可能较小，即使形成了也很容易用分子筛层析按照分子量区分开。
参与反应的两种蛋白质用大致10：1的比例混合（哪种蛋白质便宜，哪种蛋白质的物质的量就大些，但是这不是全部原因），为了避免两种蛋白质之间发生交联成为串联，因为一种蛋白质形成串联的可能性低一些；即使要形成串联的蛋白质串珠，最好也不要是价格高的那种；另外，EDC或者CMC等碳二亚胺催化活性极高，室温下即会催化，所以要在0到4度，过夜，用磁力搅拌子温和搅拌，一方面避免碳二亚胺催化活性太高形成串联的蛋白质串珠，另一方面，保护蛋白质不变性；反应样品浓度就按照平时使用时的浓度或略微更低浓度（别用储存液的浓度）即可，为什么？因为浓度高了更易形成串联的蛋白质串珠结构，浓度低了，没有反应成功，通过分子筛层析还可以分离出来接着连接，如果形成了酰胺键再要专一性切开就没有那么容易了。
