Product Specifications:
Item#1004-G: rgp160 HIV-1 IIIB Conjugated to Colloidal Gold
Concentration: See vial
Volume/vial: 1ml Gold Conjugate
Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3
Storage: 4°C
Stability: At least 3 months at 4°C Minimum
Dilution: 1 : 50
Application: Dot Blot Assay-CD4 Binding, Dot Blot Assay-Anti-gp120 Antibody Binding, Immunohistochemistry.
Description: rgp160 HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size).
Coupling Ratio: OD 520nm = 10; protein content approximately 25µg.
Specificity: Gold conjugated rgp160 binds to murine monoclonal antibodies of defined epitope specificity and human/rabbit polyclonal antibodies as determined by dot blot assays.
Biological Activity: Not determined.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. Dot blot assays may be performed in 1 : 100 dilution range depending on the concentration of solid phase CD4/Anti-gp120 in the test system.
2. Immunohistochemical studies may be performed in 1 : 50 dilution range.
Related Products:
Item#: 1004-P Peroxidase-Conjugated rgp160 HIV-1 IIIB
Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.
Item#: 1004-M Magnetically-Coupled rgp160 HIV-1 IIIB
Application: Human diagnostics, Human B and T-cell panning
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求速度考试，简答
SP法是生物素-卵白素系统：生物素为一种小分子维生素，卵白素又称为抗生物素，SP法的染色步骤为一抗～生物素化二抗～ABC复合物～DAB显色，其中ABC复合物是由一分子抗生物素蛋白与三分子结合有HRP的生物素组合成的复合物。
PV法属于非生物素系统免疫组化方法，应该是称为二步法，是以高分子的葡聚糖为载体将多个二抗分子和酶结合在一起，代替了传统的二抗和三抗，是检测变得简单而且增加了敏感性。
河南省郑州市所以食品添加剂有限公司 维生素H1价格 [未实名认证] 国产 1*20 435 元/kg
1） 物素N—羟基丁二酰亚胺酯（biotinyl-N-hydroxy-succinnimideBNHS）制备：
取物素1g混悬于12 mlN,N-二甲基甲酰胺(DMF)加0．6g N-羟基丁二酰亚胺(HOSU)0. 8 g二环基碳二亚胺(DCC)置于密闭容器内室温磁力搅拌作用夜滤滤液经旋转蒸干加10mL乙醚洗涤继加200 ml异丙醇使其重结晶获白色粉末状晶体
BNHS酯键-C=0基团与蛋白质赖氨酸氨基结合使物素标记蛋白含赖氨酸残基越或蛋白质等电点pI 6.0其标记效越BNHS适用于标记抗体及性偏碱性抗原
