ProductDetails
Source:PolyclonalRabbit
Preparation:ProducedfromseraofrabbitsimmunizedwithhighlypureRecombinantHumanVAP-1.Anti-HumanVAP-1-specificantibodywaspurifiedbyaffinitychromatographyandthenbiotinylated.
Immunogen:E.coli derivedRecombinantHumanVAP-1 (PeproTechcatalog#150-16)
SandwichELISA:TodetectHumanVAP-1bysandwichELISA(using100μl/well),aconcentrationof0.25-1.0μg/mlofthisantibodyisrequired.Thisbiotinylatedpolyclonalantibody,inconjunctionwithPeproTech’sPolyclonalAnti-HumanVAP-1(500-P326)asacaptureantibody,allowsthedetectionofatleast2000-4000pg/mlofRecombinantHumanVAP-1.
WesternBlot:TodetectHumanVAP-1byWesternBlotanalysis,thisantibodycanbeusedataconcentrationof0.1-0.2μg/ml.Whenusedinconjunctionwithcompatibledevelopmentreagents,thedetectionlimitforRecombinantHumanVAP-1is1.5-3.0ng/lane,undereitherreducingornon-reducingconditions.
Note:
1mgwillbeprovidedas2x500μg.
CountryOfOrigin: USA
Notforhumanuse.