HtrA3issecretedhumanserineproteaseissupposedtoformoligomersofidenticalproteinchainsasrevealedbyX-rayanalysisforthebacterialHtrAproteinDegP[1].PolypeptidechainsofHumanHtrA3consistsofseveraldomains:AnN-terminalinsulin-likegrowthfactordomainisfollowedbyaKazal-typeserineproteaseinhibitordomain,alinkerregion,atrypsin-likeproteasedomainandaPDZdomain[2].ThefunctionofHtrA3isprobableserineprotease.Noinformationisavailableyetonphysiologicalsubstratesorinteractingproteins.
Purity:RecombinantHtrA3appearsasamajorproteinof50kDainSDS-PAGE(>70%oftotalprotein).MinorbandsofHtrA3fragmentsmaybevisIBLeintheenzymepreparation.
EnzymaticActivity:ProteolyticactivityofrecombinanthumanHtrA3isdocumentedbydigestionofß-casein. 0.5mgß-casein/mlarecompletelydigestedby50mg/mlHtrA3within2hoursat37°C.
StABIlity&Storage:RecombinanthumanHtrA3isstableuntiltheexpirydategivenonthelabelwhenstoredat-70°C.Repeatedfreezingandthawingshouldbeavoided.
Applications:RecombinantHtrA3allowsdetailedstudiesofthestructureandfunctionsofthisprotease. Theenzymeisusedtoscreenforinhibitorsandtocharacterizeinhibitorsactions. RecombinantHtrA3canalsoserveasstandardinenzymaticandimmunochemicalassays.
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植物:抗虫、抗病、抗除草剂植物培育、改良作物品质(富含赖氨酸的玉米)
动物:提高生长速度、生产药物蛋白、器官移植、乳腺生物反应器
微生物:疫苗
基因治疗等等
各位业内前辈,我们正在考虑引进符合GMP认证标准的CHO细胞系,用于表达可做疫苗生产的重组蛋白类。目前已有符合标准信息的是Thermofisher的CHO-S悬浮培养细胞资料,希望能再多了解一些和这株细胞类似的其他公司符合GMP标准的生产株细胞做个比较。谢谢指教!
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考研细胞的题答案
生物合成人胰岛素注射液,比如诺和灵R笔芯。是单一品种、不是混合的胰岛素。是不含鱼精蛋白的短效胰岛素。

