ProductDescription:
- Produceshighqualitydendriticcells
- Yieldsareequaltoorbetterthanstandardprotocols
- Nomediumchangeoradditionrequired
- Nocytokinealiquotingrequired
- XenoandAnimal-derivedcomponentfree
- Humancellexpressedgrowthfactorsincluded
HumanKine®G4DendriticCell(DC)GenerationKithasbeendevelopedforthegrowthandgenerationofhumandendriticcells(DCs).UsingHumanKineG4DCmedium,humandendriticcellscanbeproducedfromperipheralbloodmononuclearcellsWITHOUTMEDIUMCHANGEandusedeitherasimmatureDCsorfurthercultivatedintomatureDCs.
ProtocolinBrief:
- ReconstituteCytokineCocktail
- AddCocktailtoBasalMedium
- SUSPendmonocytesincompletemedium
- Incubate7days
- DONOTCHANGEMEDIUM
- DONOTADDMEDIUM.
Linktocompleteprotocol
Specifications:
KitContents:
G4DCBasalMedium
GrowthFactorCocktail
Protocol
Kitincludesallnecessarymediumandreagents(exceptbeta-mercaptoethanolandFetalCalfSerum)
PackSize:
100mLHZ-9101
500mLHZ-9501
NOTE:A10mLculturerequiresonly10mLmedium.(plating3to5x105cells/mL)Nomediumadditionorchangeisrequired.
StorageandStABIlity:
G4DCBasalMedium:Storeat4ºC,protectfromlight.Mediumisstableforsixmonthsfromdateofproduction.
G4DCCocktail(Lyophilized):Storeat-80ºC.Cocktailisstablefortwelvemonthsfromdateofreceipt.
Completemedium(G4DCCocktailaddedtoG4DCBasalMedium):Canbestoredat4ºCforupto30daysinthedark.
QualityConfirmation:
Acertificateofanalysisisavailableonrequest.
ProductUse:
AddingtheGrowthFactorCocktailtotheG4DCBasalMediumproducesG4DendriticCellGenerationMediumrequiringnomediumadditionorchangeduringtheincubationperiod.
Theresultingcellsaremonocyte-derivedimmatureDCsthatarehighlyphagocyticwiththeimmatureDCsurfaceexpressionphenotype(CD14neg,CD83neg,CD80llow,CD86low,MHCIint,andMHCint)andcanmigrateinresponsetoCCR5agoNIST(RANTES),butnottoCCR7agonists(ELC/SLC).
PurityConfirmation:
TheproteinwasresolvedbySDSpolyacrylamidegelelectrophoresisandthegelwasstainedwithCoomassieblue.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
植物：抗虫、抗病、抗除草剂植物培育、改良作物品质（富含赖氨酸的玉米）
动物：提高生长速度、生产药物蛋白、器官移植、乳腺生物反应器
微生物：疫苗
基因治疗等等
各位业内前辈，我们正在考虑引进符合GMP认证标准的CHO细胞系，用于表达可做疫苗生产的重组蛋白类。目前已有符合标准信息的是Thermofisher的CHO-S悬浮培养细胞资料，希望能再多了解一些和这株细胞类似的其他公司符合GMP标准的生产株细胞做个比较。谢谢指教！
而没答案
帮帮我
给我提供答案
考研细胞的题答案
生物合成人胰岛素注射液，比如诺和灵R笔芯。是单一品种、不是混合的胰岛素。是不含鱼精蛋白的短效胰岛素。
暂无品牌分类
