The Antidiuretic Hormone (ADH) BioAssay™ ECL ELISA Kit (Human) is a sensitive chemiluminescent competitive enzyme immunoassay for the in vitro quantitative measurement of ADH in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.||Detection Range:|11.72-3000pg/ml||Sensitivity:|<4.66pg/ml||Precision:|Intra-Assay: CV<10%|Inter-Assay: CV<12%||Test Principle:|The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to ADH.Competitive binding is launched between a fixed amount of biotin-labeled ADH and unlabeled ADH (in the standard or sample) for limited binding sites on the pre-coated antibody. After incubation, unbound materials are washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and the plate is incubated. The addition of subtrate A and substrate B mixture generates glow light emission kinetics. The intensity of the emitted light is inversely proportional to the concentration of ADH in the sample or standard.||Kit Components:|*365717A: Microtiter Plate, 1x96 wells, Pre-coated with antibody; ready to use.|*365717B: Standard, 2x1vial |365717C: Standard Diluent, 1x20ml|*365717D: Detection Reagent A, 1x120ul |*365717E: Detection Reagent B, 1x120ul |365717F: Assay Diluent A (2x), 1x12ml|365717G: Assay Diluent B (2x), 1x12ml|365717H: Substrate A, 1x10ml|365717K: Substrate B, 1x2ml|365717L: Wash Buffer (30x), 1x20ml ||Storage and Stability:|Store *365717A, *365717B, *365717D and *365717E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Assay Procedure Summary:|1. Prepare all reagents, samples and standards.|2. Add 50ul standard or sample to each well, then add 50ul Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.|3. Aspirate and wash 3 times.|4. Add 100ul Detection Reagent B. Incubate 30 minutes at 37°C.|6. Aspirate and wash 5 times.|7. Add 100ul Substrate Solution. Incubate 10 minutes at 37°C.|8. Read RLU values immediately.|Source: Human||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

USBIo全称United States BIOLOGical，位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商，目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂，以质量**闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。

Usbio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。

USbiological公司致力于以价值，诚信和真正的个人购买体验降低研究成本。USbiological的生物化学，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。产品可以从负担得起的研究数量到大批量用于工艺开发和生产。

USBio（UnitedStatesBiological，或简称USBiological）位于麻省斯万普斯科特(Swampscott,MA)。作为全美最大的抗原抗体和生化试剂供应商，USBiological目前向全球六十余个国家的科研工作者提供约150,000种抗体、抗原和生化试剂，以质量卓越闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国USBiological的产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。USBiological一直恪守“为您的研究减少成本”原则，提供高纯度、高品质的产品，并在大多数生化试剂、生物与培养基产品线上保持最高的性价比。USBiological的产品被广泛用于各种科学研究领域，包括基因组研究、生物技术、药物研发和疾病诊断等。USBiological的产品从小型科研级到生物制品大规模级规格均有提供。众多全球顶ji的生物制药和生物技术公司都在使用USBiological的高品质产品。

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。

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