
- SpeciesReactivityHuman
- SpecificityDetectshumanCOMPindirectELISAsandWesternblots.InWesternblots,lessthan1% cross-reactivitywithrecombinanthumanThrombospondin-1, -2,-3and -4isobserved.
- SourcePolyclonalGoatIgG
- PurificationAntigenAffinity-purified
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanCOMP/Thrombospondin-5
Gln21-Ala757(Ala256Arg)
Accession#P49747 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- EndotoxinLevel<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- WesternBlot0.05µg/mLSeebelow
- NeutralizationMeasuredbyitsABIlitytoneutralizeCOMP/Thrombospondin‑5-mediatedadhesionoftheATDC5mousechondrogeniccellline.TheNeutralizationDose(ND50)istypically1-4 µg/mLinthepresenceof10 µg/mLRecombinantHumanCOMP/Thrombospondin‑5.
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Adams,J.C.andJ.Lawler(2004)IntJ.Biochem.CellBiol.36:961.
- Posey,K.L.etal.(2004)Int.J.Biochem.CellBiol.36:1005.
- Adams,J.C.(2004)Int.J.Biochem.CellBiol.36:1102.
- Mann,H.H.etal.(2004)J.Biol.Chem.279:25294.
- Newton,G.etal.(1994)Genomics,24:435.
- DiCesare,P.etal.(1995)J.OrthopaedicRes.13:422.
- Efimov,V.P.etal.(1994)FEBSLett.341:54.
- Ozbek,S.etal.(2002)EMBOJ.21:5960.
- Chen,F.H.etal.(2005)J.Biol.Chem.280:32655.
- Wislowska,M.andB.Jablonska(2005)Clin.Rheumatol.24:278.
- Xiao,Y.etal.(2004)J.Gastroenterol.Hepatol.19:296.
- Liao,Q.etal.(2003)Scand.J.Gastroenterol.38:207.
- Kennedy,J.etal.(2005)Eur.J.Hum.Genet.13:547.
- Hou,J.etal.(2000)CellCalcium27:309.
- LongName:CartilageOligomericMatrixProtein
- EntrezGeneIDs:1311(Human);12845(Mouse)
- AlternateNames:cartilageoligomericmatrixprotein(pseudoachondroplasia,epiphysealdysplasia1,multiple);cartilageoligomericmatrixprotein;cartilageoligomericmatrixprotein(pseudoachondroplasia,epiphysealdysplasia1,multiple);COMP;EDM1;EPD1;MED;MEDMGC131819;MGC149768;PSACH;pseudoachondroplasia(epiphysealdysplasia1,multiple);THBS5;Thrombospondin5;Thrombospondin-5;TSP5
Background:
CartilageOligomericMatrixProtein(COMP),alsoknownasThrombospondin-5,isa110kDamultidomaincalciumbindingproteinthatassociateswithotherextracellularmatrixmolecules.Thrombospondin-1and-2constitutesubgroupAandformhomotrimers,whereasThrombospondin-3,-4,andCOMPconstitutesubgroupBandformhomopentamers(1-4).ThehumanCOMPCDNAencodesa757aminoacid(aa)precursorthatincludesa20aasignalsequencefollowedbyanon-collagenous
coiled‑coildomain,fourEGF-likerepeats,sevenTSPtype-3repeats,andaglobularTSPC-terminaldomain(5).HumanCOMPshares86-93%aasequenceidentitywithrat,mouse,equine,bovine,andcanineCOMP.WithintheTSPtype-3repeatsandTSPC-terminaldomain,humanCOMPshares60%,61%,74%,and80%aasequenceidentitywithhumanThrombospondin-1,-2,-3,and-4,respectively.ThecoiledcoildomainmediatestheassociationofCOMPintodisulfide-linkedhomopentamerswithacentralhubandperipheralglobulardomainsconnectedbyflexIBLestrands(6, 7).AnaxialporeisformedbythecoiledcoilassemblyandbindsvitaminD3whichisinvolvedinboneandcartilagemetabolism(8).AnRGDsequenceinthethirdTSPtype-3repeatmediateschondrocyteattachmentviaIntegrinalpha5beta1,althoughwhenreducedandintheabsenceofcalcium,attachmentismediatedviaIntegrinalphaVbeta3(9).COMPisupregulatedinrheumatoidarthritisandosteoarthritis,hepatocellularcarcinomas,chronicpancreatitis,andpancreaticcarcinomas(10-12).ElevatedcirculatingCOMPlevelsareusedasabioMarkerforearlyonsetofsomeskeletaldisorders(10).Severalmutationsareassociatedwithskeletaldysplasias,andthemostcommon,apointmutationinthethirdTSPtype-3repeat,resultsindiminishedcalciumbindingability(13,14).
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