
- SpeciesReactivityHuman
- SpecificityDetectshumanCarbonicAnhydraseII/CA2indirectELISAsandWesternblots.IndirectELISAs, approximately5%cross-reactivitywithrecombinanthuman(rh)CarbonicAnhydraseI,III,and XIIIisobserved andlessthan1%cross-reactivitywithrhCarbonicAnhydraseIV,VIII,IX,X,XII,andXIVisobserved.
- SourcePolyclonalSheepIgG
- PurificationAntigenAffinity-purified
- ImmunogenE.coli-derivedrecombinanthumanCarbonicAnhydraseII/CA2
Ser2-Lys260
Accession#P00918 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot1µg/mLSeebelow
- SimpleWestern10µg/mLSeebelow
- Immunohistochemistry5-15µg/mLImmersionfixedparaffin-embeddedsectionsofhumanbrain(cerebellum)andspinalcord
- Immunoprecipitation25µg/mLConditionedcellculturemediumspikedwithRecombinantHumanCarbonicAnhydrase II(Catalog #2184-CA),seeouravailableWesternblotdetectionantibodies
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Hewett-Emmett,D.andR.E.Tashian(1996)Mol.Phylogenet.Evol.5:50.
- Shah,G.N.etal.(2004)Hum.Mutat.24:272.
- EntrezGeneIDs:760(Human)
- AlternateNames:CA2;CAII;CA-IIEC4.2.1.1;Car2;CarbonatedehydrataseII;carbonicanhydrase2;carbonicanhydraseB;CarbonicanhydraseC;CarbonicAnhydraseII;carbonicanhydraseIICAC;carbonicdehydratase
Background:
CarbonicAnhydrase(CA)catalyzesthereversIBLereactionofCO2+H2O=HCO3-+H+,whichisfundamentaltomanyprocessessuchasrespiration,renaltubularacidificationandboneresorption(1).Topicsinthe CAmeeting(6thInternationalConferenceontheCAs,June20‑25,2003,Slovakia)rangedfromuseofCAsasMarkersfortumorandhypoxiaintheclinic,asnutritionalsupplementinmilk,andasatoolforCO2removalandmosquitocontrolinindustry.CA2isacytosolicenzymewiththehighestactivityamongallknownCAs.MutationsintheCA2generesultintheCAIIdeficiencysyndrome,anautosomalrecessivedisorderthatproducesosteopetrosis,renaltubularacidosisandcerebralcalcification(2).
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